Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway

被引：47
作者：
Chen, Fenglin [1 ]
Zhuang, Mingkai [2 ]
Peng, Jun [3 ]
Wang, Xiaozhong [1 ]
Huang, Tingxuan [4 ]
Li, Sanmei [1 ]
Lin, Manqiang [1 ]
Un, Hongming [1 ]
Xu, Yating [1 ]
Li, Jianying [1 ]
Chen, Zhixin [1 ]
Huang, Yuehong [1 ]
机构：
[1] Fujian Med Univ, Union Hosp, Dept Gastroenterol, Fuzhou 350001, Fujian, Peoples R China
[2] Fujian Med Univ, Coll Union Clin Med, Fuzhou 350001, Fujian, Peoples R China
[3] Fujian Univ Tradit Chinese Med, Acad Integrat Med Biomed Res Ctr, Fuzhou 350001, Fujian, Peoples R China
[4] Fujian Med Univ, Coll Basic Med Sci, Fuzhou 350001, Fujian, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2014年 / 10卷 / 04期
关键词：
baicalein; gastric carcinoma; neoplasm metastasis; transforming growth factor-beta; GROWTH-FACTOR; TRANSFORMING GROWTH-FACTOR-BETA-1; MESENCHYMAL TRANSITION; E-CADHERIN; DIFFERENTIATION; INVASIVENESS; SCUTELLARIA; METASTASIS; CARCINOMA; ADHESION;
D O I：
10.3892/mmr.2014.2452
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The transforming growth factor-beta (TGF-beta) signaling pathway exhibits an important role in cancer invasion and metastasis. Excessive expression of TGF-beta activates Smad4, leading to the upregulation of downstream metastasis-associated genes. Thus, the inhibition of the TGF-beta/Smad4 signaling pathway may be a novel strategy for treatment of cancer metastasis. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis, has been reported to exert strong anti-tumor activity towards various types of cancer. In the present study the effect of baicalein on migration and invasion of cancer cells was evaluated using wound-healing and Transwell assays. In order to investigate the possible molecular mechanisms of the anti-metastatic effects of baicalein, quantitative polymerase chain reaction (qPCR) and western blot analyses were performed to examine the effect on the expression of TGF-beta; Smad4, N-cadherin, vimentin, ZEB1 and ZEB2 It was determined that baicalein inhibited the migration and invasion of AGS cells by suppressing the TGF-beta/Smad4 signaling pathway. In addition, baicalein treatment reduced the expression of the metastasis-associated N-cadherin, vimentin, ZEB1 and ZEB2, downstream target genes of the TGF-P/Smad4 signaling pathway. Collectively, these results suggest that inhibition of the metastasis of cancer cells via inactivation of TGF-beta/Smad4 signaling is one of the mechanisms by which baicalein may treat cancer.
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收藏
页码：1999 / 2003
页数：5
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