Reduced expressions of Foxp1 and Rassf1a genes in lung adenocarcinornas induced by N-nitrosobis(2-hydroxypropyl) amine in rats

被引：11

作者：

Shimizu, Kyoko

Kato, Ayumi

Hinotsume, Daisei

Shigemura, Mayumi

Hanaoka, Madoka

Shimoichi, Yozo

Honoki, Kanya

Tsujiuchi, Toshifumi

机构：

[1] Kinki Univ, Lab Canc Biol & Bioinformat, Dept Life Sci, Fac Sci & Technol, Higashiosaka, Osaka 5778502, Japan

[2] Nara Med Univ, Dept Orthoped Surg, Kashihara, Nara 6348521, Japan

来源：

CANCER LETTERS | 2006年 / 236卷 / 02期

关键词：

Foxp1; Rassf1a; lung adenocarcinoma; rat; nitrosamine;

D O I：

10.1016/j.canlet.2005.05.031

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To clarify the involvement of the Foxp1 and Rassf1a genes in lung carcinogenesis, we investigated their expressions in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in rats. Six week old male Wistar rats were given 2000 ppm BHP in their drinking water for 12 weeks and maintained without further treatment until they were sacrificed at 25 weeks. A total of 10 lung adenocarcinomas were obtained, along with the total RNA from each for assessment of expression by reverse transcription (RT)-polymerase chain reaction (PCR). The reduced expressions of the Foxp1 and Rassf1a genes were observed in some of the lung adenocarcinomas. These analyses were also confirmed by real-time quantitative RT-PCR. These results suggest that reduced expressions of Foxp1 and Rassf1a genes may play a role in the development of lung adenocarcinomas induced by BHP in rats. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：186 / 190

页数：5

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