An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells

被引：42

作者：

Duan, Qinya ^{[1
]}

Zheng, Guansheng ^{[1
]}

Li, Zejun ^{[1
]}

Cheng, Ke ^{[2
,3
,4
]}

Zhang, Jie ^{[2
,3
,4
]}

Yang, Liu ^{[2
,3
,4
]}

Jiang, Yin ^{[1
]}

Zhang, Huatang ^{[1
]}

He, Jun ^{[1
]}

Sun, Hongyan ^{[2
,3
,4
]}

机构：

[1] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China

[2] City Univ Hong Kong, Dept Chem, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China

[3] City Univ Hong Kong, COSDAF, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China

[4] City Univ Hong Kong, Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen Res Inst, Shenzhen 518057, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2019年 / 7卷 / 33期

基金：

中国国家自然科学基金;

关键词：

LIVE-CELL; FAR-RED; MYELOPEROXIDASE; MECHANISMS; CANCER; MITOCHONDRIA; OXIDATION; DESIGN; ROLES; WATER;

D O I：

10.1039/c9tb01279f

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I-589 nm/I-477 nm), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Lastly, the successful result of exogenous/endogenous HOCl imaging detection in different cell lines indicates the potential use of the probe in living systems.

引用

页码：5125 / 5131

页数：7