ZAK negatively regulates RhoGDIβ-induced Rac1-mediated hypertrophic growth and cell migration

被引：11

作者：

Huang, Chih-Yang ^{[2
,3
,4
]}

Yang, Li-Chiu ^{[1
,5
,6
]}

Liu, Kuan-Yu ^{[1
]}

Chang, I-Chang ^{[7
]}

Liao, Pao-Hsin ^{[1
,6
]}

Chou, Janet Ing-Yuh ^{[8
]}

Chou, Ming-Yung ^{[1
,6
]}

Lin, Wei-Wen ^{[9
]}

Yang, Jaw-Ji ^{[1
]}

机构：

[1] Chung Shan Med Univ, Sch Dent, Taichung 402, Taiwan

[2] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan

[3] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan

[4] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan

[5] Chung Shan Med Univ, Inst Med, Taichung 402, Taiwan

[6] Chung Shan Med Univ, Chung Shan Med Univ Hosp, Taichung 402, Taiwan

[7] Chung Shan Med Univ, Dept Orthoped Surg, Taichung 402, Taiwan

[8] Midwestern Univ Chicago, Coll Osteopath Med, Chicago, IL USA

[9] Vet Gen Hosp, Ctr Cardiovasc, Taichung 407, Taiwan

来源：

JOURNAL OF BIOMEDICAL SCIENCE | 2009年 / 16卷

关键词：

CARDIOMYOBLAST CELLS; GTPASES; EXPRESSION; KINASE; GDI; RAC; PATHWAYS; ADHESION; PROTEIN;

D O I：

10.1186/1423-0127-16-56

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RhoGDI beta, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDI beta plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDI beta expression by RNA interference blocked RhoGDI beta-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDI beta in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDI beta-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDI beta.

引用

页数：9

共 22 条

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