Long non-coding RNA DLX6-AS1 silencing inhibits malignant phenotypes of gastric cancer cells

被引：22
作者：
Fu, Xiaodan [1 ]
Tian, Yifu [1 ]
Kuang, Weilu [2 ]
Wen, Sailan [3 ]
Guo, Wei [4 ]
机构：
[1] Cent South Univ, Xiangya Hosp, Dept Pathol, 87 Xiangya Rd, Changsha 410005, Hunan, Peoples R China
[2] Cent South Univ, Xiangya Hosp, Dept Oncol, Changsha 410005, Hunan, Peoples R China
[3] Cent South Univ, Xiangya Hosp 2, Dept Pathol, Changsha 410011, Hunan, Peoples R China
[4] Hunan Normal Univ, Dept Pathol, Hunan Prov Peoples Hosp, Affiliated Hosp 1, Changsha 410002, Hunan, Peoples R China
来源：
EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2019年 / 17卷 / 06期
关键词：
long non-coding RNA; distal-less homeobox 6 antisense 1; gastric cancer; prognosis; EPITHELIAL-MESENCHYMAL TRANSITION; PROMOTES; PROGRESSION; EXPRESSION; INVASION; NEAT1;
D O I：
10.3892/etm.2019.7521
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
It has been revealed that long non-coding RNAs (lncRNAs) serve a key role in various malignancies, including gastric cancer (GC). In the present study, the expression and function of lncRNA distal-less homeobox 6 antisense 1 (DLX6-AS1) in GC was investigated. The data revealed that the expression of DLX6-AS1 was significantly upregulated in GC tissues compared with adjacent paired noncancerous tissues. Furthermore, the expression of DLX6-AS1 was higher in advanced GC tissue samples (III/IV) compared with the expression in early-stage samples (I/II). Furthermore, the current study demonstrated that a high expression of DLX6-AS1 was significantly associated with advanced clinical stage, lymph node metastasis and distant metastasis. Compared with patients with a low DLX6-AS1 expression, DLX6-AS1 expression in patients with GC was associated with decreased survival. In vitro experimental data indicated that DLX6-AS1 was upregulated in GC cell lines and that the inhibition of DLX6-AS1 markedly reduced GC cell proliferation, colony formation, cell cycle progression, migration and invasion. Further investigation revealed that knockdown of DLX6-AS1 inhibited EMT in GC cells. In summary, the present study demonstrated that lncRNA DLX6-AS1 was upregulated and serves an oncogenic role in GC, indicating that DLX6-AS1 may be a novel therapeutic target for GC treatment.
引用
收藏
页码：4715 / 4722
页数：8
相关论文
共 35 条
[1]   The long noncoding RNA, TINCR, functions as a competing endogenous RNA to regulate PDK1 expression by sponging miR-375 in gastric cancer [J].
Chen, Zhaoliang ;
Liu, Hong ;
Yang, Huili ;
Gao, Yukai ;
Zhang, Gongwen ;
Hu, Jiaojiao .
ONCOTARGETS AND THERAPY, 2017, 10 :3353-3361
[2]   Long noncoding RNAs in cell biology [J].
Clark, Michael B. ;
Mattick, John S. .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2011, 22 (04) :366-376
[3]   Long noncoding RNA NEAT1 is an unfavorable prognostic factor and regulates migration and invasion in gastric cancer [J].
Fu, Jing-wei ;
Kong, Ying ;
Sun, Xu .
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2016, 142 (07) :1571-1579
[4]   The functional role of long non-coding RNA in human carcinomas [J].
Gibb, Ewan A. ;
Brown, Carolyn J. ;
Lam, Wan L. .
MOLECULAR CANCER, 2011, 10
[5]   Long noncoding RNA in genome regulation Prospects and mechanisms [J].
Hung, Tiffany ;
Chang, Howard Y. .
RNA BIOLOGY, 2010, 7 (05) :582-585
[6]  
Jemal A, 2011, CA-CANCER J CLIN, V61, P134, DOI [10.3322/caac.20115, 10.3322/caac.21492, 10.3322/caac.20107]
[7]   RETRACTED: The silencing of LncRNA-H19 decreases chemoresistance of human glioma cells to temozolomide by suppressing epithelial-mesenchymal transition via the Wnt/β-Catenin pathway (Retracted Article) [J].
Jia, Linwei ;
Tian, Yaohui ;
Chen, Yonghan ;
Zhang, Gang .
ONCOTARGETS AND THERAPY, 2018, 11 :313-321
[8]   Exosomes: from carcinogenesis and metastasis to diagnosis and treatment of gastric cancer [J].
Kahroba, Houman ;
Hejazi, Mohammad Saeid ;
Samadi, Nasser .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2019, 76 (09) :1747-1758
[9]   Long non-coding RNA XIST promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-367/141-ZEB2 axis in non-small-cell lung cancer [J].
Li, Chang ;
Wan, Liang ;
Liu, Zeyi ;
Xu, Guangquan ;
Wang, Shengjie ;
Su, Zhiyue ;
Zhang, Yingxi ;
Zhang, Cuijuan ;
Liu, Xia ;
Lei, Zhe ;
Zhang, Hong-Tao .
CANCER LETTERS, 2018, 418 :185-195
[10]   Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma [J].
Li, Juan ;
Li, Ping ;
Zhao, Wei ;
Yang, Rui ;
Chen, Shanshan ;
Bai, Yong ;
Dun, Shaozhi ;
Chen, Xiaonan ;
Du, Yuwen ;
Wang, Yuanyuan ;
Zang, Wenqiao ;
Zhao, Guoqiang ;
Zhang, Guojun .
CANCER CELL INTERNATIONAL, 2015, 15
1234