PHYLOGENETIC CHARACTERIZATION OF POTENTIAL BIOACTIVE METABOLITES PRODUCING ACTINOMYCETES FROM MANGROVE SEDIMENTS

被引：0
作者：
Naragani, Krishna [1 ]
Indupalli, Mani Deepa [2 ]
Muvva, Vijayalakshmi [3 ]
机构：
[1] PB Siddhartha Coll Arts & Sci, Dept Bot, Vijayawada 520010, Andhra Pradesh, India
[2] Maris Stella Coll, Dept Microbiol, Vijayawada 520008, Andhra Pradesh, India
[3] Acharya Nagarjuna Univ, Dept Bot & Microbiol, Guntur 522510, Andhra Pradesh, India
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH | 2020年 / 11卷 / 12期
关键词：
Mangrove ecosystem; Microbial diversity; Phylogenetic analysis and Antimicrobial activity;
D O I：
10.13040/IJPSR.0975-8232.11(12).6124-29
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
An attempt has been made in the present study to isolate and identify actinomycetes, which can produce bioactive metabolites possessing antimicrobial activity. The soil samples were collected from mangrove sediments of Krishna district. Andhra Pradesh. The potent secondary metabolite producing strains were isolated and designated as VLK-15, VLK-24, and VLK-56. Identification of the strains was carried out basing on morphological, cultural, physiological and biochemical characters. Phylogenetic analysis of 16S rRNA gene sequence showed that the strains VLK-15, VLK-24, VLK-56 form distinct Glade within the genus Streptomyces. The 16S rRNA sequences were deposited in the GenBank database of NCBI under the accession numbers MG309759 (Streptomyces olivoverticillatus VLK-15), MG309760 (Strepto-myces rubrus VLK-24), and MF952650 (Streptomyces sampsonii-VLK-56). The antimicrobial activity of the strains was evaluated by using agar well diffusion assay. The ethyl acetate extracts were highly effective against Candida albicans, Staphylococcus aureus. Bacillus megaterium followed by Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae.
引用
收藏
页码：6124 / 6129
页数：6
相关论文
共 17 条
[1]   Production of Antimicrobial Metabolites by Streptomyces lavendulocolor VHB-9 Isolated from Granite Mines [J].
Bindu, Hima B. S. S. N. ;
Muvva, Vijayalakshmi ;
Munaganti, Rajesh Kumar ;
Naragani, Krishna ;
Konda, Saidulu ;
Dorigondla, Kumar Reddy .
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2017, 60
[2]  
Gupte S, 2017, J BACTERIOL MYCOL, V4
[3]  
Hensy WR, 1994, BERGEYS MANUAL SYSTE
[4]   Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China [J].
Hong, Kui ;
Gao, An-Hui ;
Xie, Qing-Yi ;
Gao, Hao ;
Zhuang, Ling ;
Lin, Hai-Peng ;
Yu, Hai-Ping ;
Li, Jia ;
Yao, Xin-Sheng ;
Goodfellow, Michael ;
Ruan, Ji-Sheng .
MARINE DRUGS, 2009, 7 (01) :24-44
[5]   Bioactive compounds from mangrove derived rare actinobacterium Saccharomonospora oceani VJDS-3 [J].
Indupalli, Manideepa ;
Muvva, Vijayalakshmi ;
Mangamuri, Ushakiranmayi ;
Munaganti, Rajesh Kumar ;
Naragani, Krishna .
3 BIOTECH, 2018, 8
[6]   Unexplored hypersaline habitats are sources of novel actinomycetes [J].
Jose, Polpass Arul ;
Jebakumar, Solomon Robinson David .
FRONTIERS IN MICROBIOLOGY, 2014, 5
[7]  
Kiranmayi M. U., 2011, Journal of Pharmacy Research, V4, P4650
[8]   MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms [J].
Kumar, Sudhir ;
Stecher, Glen ;
Li, Michael ;
Knyaz, Christina ;
Tamura, Koichiro .
MOLECULAR BIOLOGY AND EVOLUTION, 2018, 35 (06) :1547-1549
[9]   Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity [J].
Mangamuri, Ushakiranmayi ;
Muvva, Vijayalakshmi ;
Poda, Sudhakar ;
Naragani, Krishna ;
Munaganti, Rajesh Kumar ;
Chitturi, Bhujangarao ;
Yenamandra, Venkateswarlu .
3 BIOTECH, 2016, 6 :1-8
[10]   Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards [J].
Munaganti, Rajesh Kumar ;
Muvva, Vijayalakshmi ;
Konda, Saidulu ;
Naragani, Krishna ;
Mangamuri, Usha Kiranmayi ;
Dorigondla, Kumar Reddy ;
Akkewar, Dattatray M. .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2016, 47 (04) :1030-1038
12