Tyrosine dephosphorylation regulates AMPAR internalisation in mGluR-LTD

被引:51
作者
Gladding, Clare M. [1 ]
Collett, Valerie J. [1 ]
Jia, Zhengping [2 ]
Bashir, Zafar I. [1 ]
Collingridge, Graham L. [1 ]
Molnar, Elek [1 ]
机构
[1] Univ Bristol, Sch Med Sci, Dept Anat, Med Res Council Ctr Synapt Plastic, Bristol BS8 1TD, Avon, England
[2] Univ Toronto, Hosp Sick Children, Neurosci & Mental Hlth Program, Toronto, ON M5G 1X8, Canada
基金
英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金; 加拿大健康研究院;
关键词
LONG-TERM DEPRESSION; ACTIVATED PROTEIN-KINASE; DHPG-INDUCED LTD; METABOTROPIC GLUTAMATE RECEPTORS; HIPPOCAMPAL CA1 REGION; AREA CA1; SYNAPTIC PLASTICITY; POSTSYNAPTIC INDUCTION; PRESYNAPTIC EXPRESSION; RAPID REDISTRIBUTION;
D O I
10.1016/j.mcn.2008.10.014
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Long-term depression (LTD) can be induced at hippocampal CA1 synapses by activation of either NMDA receptors (NMDARs) or group I metabotropic glutamate receptors (mGluRs), using their selective agonists NMDA and (RS)-3,5-dihydroxyphenylglycine(DHPG), respectively. Recent studies revealed that DHPC-LTD is dependent on activation of postsynaptic protein tyrosine phosphatases (PTPs), which transiently dephosphorylate tyrosine residues in AMPA receptors (AMPARs). Here we show that while both endogenous GluR2 and GluR3 AMPAR Subunits are tyrosine phosphorylated at basal activity, only GluR2 is dephosphorylated in DHPG-LTD. The tyrosine dephosphorylation of GluR2 does not occur in NMDA-LTD. Conversely, while NMDA-LTD is associated with the dephosphorylation of GluR1-serine-845, DHPG-LTD does not alter the phosphorylation of this site. The increased AMPAR endocytosis in DHPG-LTD is PTP-dependent and involves tyrosine dephosphorylation of cell Surface AMPARs. Together, these results indicate that the Subunit selective tyrosine dephosphorylation of surface GluR2 regulates AMPAR internalisation in DHPG-LTD but not in NMDA-LTD in the hippocampus. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:267 / 279
页数:13
相关论文
共 68 条
  • [1] Tyrosine phosphorylation of GluR2 is required for insulin-stimulated AMPA receptor endocytosis and LTD
    Ahmadian, G
    Ju, W
    Liu, LD
    Wyszynski, M
    Lee, SH
    Dunah, AW
    Taghibiglou, C
    Wang, YS
    Lu, J
    Wong, TP
    Sheng, M
    Wang, YT
    [J]. EMBO JOURNAL, 2004, 23 (05) : 1040 - 1050
  • [2] Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs
    Ashby, MC
    De La Rue, SA
    Ralph, GS
    Uney, J
    Collingridge, GL
    Henley, JM
    [J]. JOURNAL OF NEUROSCIENCE, 2004, 24 (22) : 5172 - 5176
  • [3] Lateral diffusion drives constitutive exchange of AMPA receptors at dendritic spines and is regulated by spine morphology
    Ashby, Michael C.
    Maier, Susie R.
    Nishimune, Atsushi
    Henley, Jeremy M.
    [J]. JOURNAL OF NEUROSCIENCE, 2006, 26 (26) : 7046 - 7055
  • [4] METABOTROPIC GLUTAMATE RECEPTORS CONTRIBUTE TO THE INDUCTION OF LONG-TERM DEPRESSION IN THE CA1 REGION OF THE HIPPOCAMPUS
    BASHIR, ZI
    JANE, DE
    SUNTER, DC
    WATKINS, JC
    COLLINGRIDGE, GL
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 239 (1-3) : 265 - 266
  • [5] Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD
    Beattie, EC
    Carroll, RC
    Yu, X
    Morishita, W
    Yasuda, H
    von Zastrow, M
    Malenka, RC
    [J]. NATURE NEUROSCIENCE, 2000, 3 (12) : 1291 - 1300
  • [6] Dynamics and regulation of clathrin coats at specialized endocytic zones of dendrites and spines
    Blanpied, TA
    Scott, DB
    Ehlers, MD
    [J]. NEURON, 2002, 36 (03) : 435 - 449
  • [7] POSTSYNAPTIC INDUCTION AND PRESYNAPTIC EXPRESSION OF HIPPOCAMPAL LONG-TERM DEPRESSION
    BOLSHAKOV, VY
    SIEGELBAUM, SA
    [J]. SCIENCE, 1994, 264 (5162) : 1148 - 1152
  • [8] Role of AMPA receptor endocytosis in synaptic plasticity
    Carroll, RC
    Beattie, EC
    von Zastrow, M
    Malenka, RC
    [J]. NATURE REVIEWS NEUROSCIENCE, 2001, 2 (05) : 315 - 324
  • [9] Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures
    Carroll, RC
    Lissin, DV
    von Zastrow, M
    Nicoll, RA
    Malenka, RC
    [J]. NATURE NEUROSCIENCE, 1999, 2 (05) : 454 - 460
  • [10] Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression
    Chung, HJ
    Steinberg, JP
    Huganir, RL
    Linden, DJ
    [J]. SCIENCE, 2003, 300 (5626) : 1751 - 1755