共 68 条
Tyrosine dephosphorylation regulates AMPAR internalisation in mGluR-LTD
被引:51
作者:
Gladding, Clare M.
[1
]
Collett, Valerie J.
[1
]
Jia, Zhengping
[2
]
Bashir, Zafar I.
[1
]
Collingridge, Graham L.
[1
]
Molnar, Elek
[1
]
机构:
[1] Univ Bristol, Sch Med Sci, Dept Anat, Med Res Council Ctr Synapt Plastic, Bristol BS8 1TD, Avon, England
[2] Univ Toronto, Hosp Sick Children, Neurosci & Mental Hlth Program, Toronto, ON M5G 1X8, Canada
基金:
英国生物技术与生命科学研究理事会;
英国医学研究理事会;
英国惠康基金;
加拿大健康研究院;
关键词:
LONG-TERM DEPRESSION;
ACTIVATED PROTEIN-KINASE;
DHPG-INDUCED LTD;
METABOTROPIC GLUTAMATE RECEPTORS;
HIPPOCAMPAL CA1 REGION;
AREA CA1;
SYNAPTIC PLASTICITY;
POSTSYNAPTIC INDUCTION;
PRESYNAPTIC EXPRESSION;
RAPID REDISTRIBUTION;
D O I:
10.1016/j.mcn.2008.10.014
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
Long-term depression (LTD) can be induced at hippocampal CA1 synapses by activation of either NMDA receptors (NMDARs) or group I metabotropic glutamate receptors (mGluRs), using their selective agonists NMDA and (RS)-3,5-dihydroxyphenylglycine(DHPG), respectively. Recent studies revealed that DHPC-LTD is dependent on activation of postsynaptic protein tyrosine phosphatases (PTPs), which transiently dephosphorylate tyrosine residues in AMPA receptors (AMPARs). Here we show that while both endogenous GluR2 and GluR3 AMPAR Subunits are tyrosine phosphorylated at basal activity, only GluR2 is dephosphorylated in DHPG-LTD. The tyrosine dephosphorylation of GluR2 does not occur in NMDA-LTD. Conversely, while NMDA-LTD is associated with the dephosphorylation of GluR1-serine-845, DHPG-LTD does not alter the phosphorylation of this site. The increased AMPAR endocytosis in DHPG-LTD is PTP-dependent and involves tyrosine dephosphorylation of cell Surface AMPARs. Together, these results indicate that the Subunit selective tyrosine dephosphorylation of surface GluR2 regulates AMPAR internalisation in DHPG-LTD but not in NMDA-LTD in the hippocampus. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.
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页码:267 / 279
页数:13
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