Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor

被引:37
作者
Donia, Marco [1 ,2 ,3 ]
Larsen, Signe Mollebaek [1 ]
Met, Ozcan [1 ,2 ]
Svane, Inge Marie [1 ,2 ]
机构
[1] Herlev Hosp, Ctr Canc Immune Therapy, Dept Hematol, DK-2730 Herlev, Denmark
[2] Herlev Hosp, Dept Oncol, DK-2730 Herlev, Denmark
[3] Univ Catania, Dept Biomed Sci, I-95124 Catania, Italy
关键词
metastatic melanoma; rapid expansion; tumor-infiltrating lymphocytes; Wave bioreactor; ADOPTIVE CELL THERAPY; EXPANSION; IMMUNOTHERAPY; CULTURES;
D O I
10.1016/j.jcyt.2014.02.004
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background aims. The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs). Methods. To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution. Results. This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma. Conclusions. Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
引用
收藏
页码:1117 / 1120
页数:4
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