Aestuariimonas insulae gen. nov., sp nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-44(T), was isolated from a tidal flat in Oido, an island of the Republic of Korea. Strain OITF-44(T) grew optimally at 25 degrees C and in the presence of 2.0% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-44(T) formed an independent lineage within the clade comprising the genera Lutimonas, Taeania, Actibacter and Namhaeicola. The novel strain exhibited 16S rRNA gene sequence similarity values of 93.9-95.7% to the type strains of the species of the genera Lutimonas, Taeania, Actibacter and Namhaeicola, and of less than 93.5% to the type strains of other recognized species. Strain OITF-44(T) contained MK-6 as the predominant menaquinone and iso-C-15 : 0, iso-C-15 : 1 G, iso-C-16 : 1 H and iso-C-16 : 03-OH as the major fatty acids. The major polar lipids of strain OITF-44(T) were phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content of strain OITF-44(T) was 33.9 mol%. The chemotaxonomic data and other differential phenotypic properties made it reasonable to distinguish strain OITF-44(T) from the type strains of the type species of the genera Lutimonas, Taeania, Actibacter and Namhaeicola. On the basis of the data presented here, strain OITF-44(T) is considered to constitute a new genus and species within the family Flavobacteriaceae, for which the name Aestuariimonas insulae gen. nov., sp. nov. is proposed. The type strain of Aestuariimonas insulae is OITF-44(T) (= KACC 19569(T) = KCTC 62197(T) = DSM 105891(T) = NBRC 113118(T)).