A nanosecond laser fluorimeter

被引:4
作者
Banishev, AA [1 ]
Maslov, DV [1 ]
Fadeev, VV [1 ]
机构
[1] Moscow MV Lomonosov State Univ, Moscow 119992, Russia
基金
俄罗斯基础研究基金会;
关键词
78.47.+p; 42.62.Fi; 78.55.Bq;
D O I
10.1134/S0020441206030237
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
A method for determining the fluorescence decay times in the range 1-10 ns using a nanosecond laser fluorimeter is described. The fluorimeter has the following performance characteristics: a width of exciting pulses of 10 ns, a width of gate pulses of 10 ns, and a step of gate-pulse displacement of 2.5 ns. It is shown that this instrument allows determination of fluorescence decay times with an accuracy of 1-1.5 ns.
引用
收藏
页码:430 / 434
页数:5
相关论文
共 8 条
[1]  
BANISHEV AA, 2005, PHYS PROBLEMS ECOLOG, P138
[2]  
Birks Dzh., 1971, USP FIZ NAUK, V105, P251
[3]   Time-resolved fluorimetry of two-fluorophore organic systems using artificial neural networks [J].
Dolenko, SA ;
Dolenko, TA ;
Fadeev, VV ;
Gerdova, IV ;
Kompitsas, M .
OPTICS COMMUNICATIONS, 2002, 213 (4-6) :309-324
[4]   Saturation spectroscopy as a method for determining the photophysical parameters of complicated organic compounds [J].
Fadeev, VV ;
Dolenko, TA ;
Filippova, EM ;
Chubarov, VV .
OPTICS COMMUNICATIONS, 1999, 166 (1-6) :25-33
[5]  
GALANIN MD, 1979, P 3 C LUM, P3
[6]   RESOLUTION OF MIXTURES OF FLUOROPHORES USING VARIABLE-FREQUENCY PHASE AND MODULATION DATA [J].
GRATTON, E ;
LIMKEMAN, M ;
LAKOWICZ, JR ;
MALIWAL, BP ;
CHEREK, H ;
LACZKO, G .
BIOPHYSICAL JOURNAL, 1984, 46 (04) :479-486
[7]   A subnanosecond time-resolved fluorescence lifetime spectrometer applying laser diodes [J].
Landgraf, S ;
Grampp, G .
MONATSHEFTE FUR CHEMIE, 2000, 131 (08) :839-848
[8]   GLOBAL TARGET ANALYSIS OF PICOSECOND CHLOROPHYLL FLUORESCENCE KINETICS FROM PEA-CHLOROPLASTS - A NEW APPROACH TO THE CHARACTERIZATION OF THE PRIMARY PROCESSES IN PHOTOSYSTEM-II ALPHA-UNITS AND BETA-UNITS [J].
ROELOFS, TA ;
LEE, CH ;
HOLZWARTH, AR .
BIOPHYSICAL JOURNAL, 1992, 61 (05) :1147-1163