An improved Escherichia coli donor strain for diparental mating

被引：159

作者：

Thoma, Sabrina ^{[1
]}

Schobert, Max ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, D-38106 Braunschweig, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 294卷 / 02期

关键词：

diparental mating; Escherichia coli; hemA; heme biosynthesis; BROAD-HOST-RANGE; 5-AMINOLEVULINIC ACID SYNTHESIS; GRAM-NEGATIVE BACTERIA; PSEUDOMONAS-AERUGINOSA; RECOMBINATION SYSTEM; PROTEIN; GENE; ELECTROPORATION; TRANSFORMATION; IDENTIFICATION;

D O I：

10.1111/j.1574-6968.2009.01556.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present a new method for diparental mating with the outstanding advantage that counterselection of the Escherichia coli donor strain is not required. This improved method uses a new donor strain, E. coli ST18, a hemA deletion mutant defective in tetrapyrrole biosynthesis. The hemA mutation can be complemented by addition of 5-aminolevulinic acid. Therefore, counterselection is carried out only using standard media and growth conditions optimal for the recipient strain. Consequently, recipient strains are isolated in a significantly shorter period.

引用

页码：127 / 132

页数：6

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