Ceramides and sphingomyelinases in senile plaques

被引：50

作者：

Panchal, Mai ^{[1
,2
]}

Gaudin, Mathieu ^{[3
,4
]}

Lazar, Adina N. ^{[1
,2
,5
]}

Salvati, Elisa ^{[6
,7
]}

Rivals, Isabelle ^{[8
]}

Ayciriex, Sophie ^{[3
]}

Dauphinot, Luce ^{[2
]}

Dargere, Delphine ^{[3
]}

Auzeil, Nicolas ^{[3
]}

Masserini, Massimo ^{[6
,7
]}

Laprevote, Olivier ^{[3
,9
]}

Duyckaerts, Charles ^{[1
,2
,5
]}

机构：

[1] Hop La Pitie Salpetriere, AP HP, Lab Neuropathol Escourolle, F-75651 Paris 13, France

[2] UPMC, INSERM UMR S 975, CNRS UMR 7225, Ctr Rech ICM, F-75654 Paris 13, France

[3] Univ Paris 05, PRES Sorbonne Paris Cite, Fac Sci Pharmaceut & Biol, EA 4463, Paris, France

[4] Technol Servier, Div Metab, Orleans, France

[5] UPMC Paris Univ, Paris, France

[6] Univ Milano Bicocca, Dept Hlth Sci, Monza, Italy

[7] Univ Milano Bicocca, Nanomed Ctr, Monza, Italy

[8] ESPCI Paris Tech, Equipe Stat Appl, Paris, France

[9] Hop Lariboisiere, AP HP, Lab Toxicol Biol, F-75475 Paris, France

来源：

NEUROBIOLOGY OF DISEASE | 2014年 / 65卷

关键词：

Alzheimer disease; Senile plaques; Laser microdissection; Lipidomics; Mass spectrometry; Ceramides; Lipids; A beta peptide; Sphingomyelinase; ALZHEIMERS-DISEASE; AMYLOID-BETA; SPHINGOLIPID METABOLISM; APOLIPOPROTEIN-E; CHOLESTEROL; BRAIN; INVOLVEMENT; ASSOCIATION; EXPRESSION; APOPTOSIS;

D O I：

10.1016/j.nbd.2014.01.010

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The senile plaque is a hallmark lesion of Alzheimer disease (AD). We compared, without a priori, the lipidome of the senile plaques and of the adjacent plaque-free neuropil. The analysis by liquid chromatography coupled with electrospray ionization mass spectrometry revealed that laser microdissected senile plaques were enriched in saturated ceramides Cer(d18:1/18:0) and Cer(d18:1/20:0) by 33 and 78% respectively with respect to the surrounding neuropil. This accumulation of ceramides was not explained by their affinity for A beta deposits: no interaction between ceramide-liposomes and A beta fibrils was observed in vitro by surface plasmon resonance and fluorescent ceramide-liposomes showed no affinity for the senile plaques in AD brain tissue. Accumulation of ceramides could be, at least partially, the result of a local production by acid and neutral sphingomyelinases that we found to be present in the corona of the senile plaques. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：193 / 201

页数：9

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