Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer

被引:12
作者
Wu, Haiyun [1 ]
Fujii, Yuzu [1 ]
Nakano, Toshiki [2 ]
Arimoto, Takafumi [1 ]
Murata, Masataka [3 ]
Matsumoto, Haruto [3 ]
Yoshiura, Yasutoshi [4 ]
Ohnuki, Hitoshi [1 ]
Endo, Hideaki [1 ]
机构
[1] Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Tokyo 1088477, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9808572, Japan
[3] Hokkaido Ind Technol Ctr, Hakodate, Hokkaido 0410801, Japan
[4] Japan Fisheries Res & Educ Agcy, Natl Res Inst Fisheries & Environm Inland Sea, Takamatsu, Kagawa 7610111, Japan
关键词
biosensor; real-time monitoring; fish; stress; self-assembled monolayer; glucose; GILTHEAD SEA BREAM; SPARUS-AURATA L; PHYSIOLOGICAL-RESPONSES; BLOOD-GLUCOSE; IMMUNOSENSOR; PROTEIN;
D O I
10.3390/s19071518
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Wireless biosensor systems were developed in our lab for monitoring blood glucose concentrations in fish as an indicator of fish stress. However, uniform immobilization of the enzyme on the surface of the electrode is difficult, so the sensor response is typically reduced at a range of high glucose concentrations during the stress monitoring. In this study, we attempted to enhance sensor response by using a self-assembled monolayer-immobilized enzyme. Glucose oxidase was immobilized on a working electrode modified with a self-assembled monolayer. The proposed biosensor showed a good correlation between the output current and the glucose concentration range of 10-3500 mg dL(-1) under an optimized working condition. The dynamic measurement range of this newly developed sensor is significantly improved, especially over a high concentration range, which helps the sensor to achieve better performance in dramatic changes in the stress response of fish. In addition, we used biological samples from test fish and obtained a good correlation coefficient between the sensor output current and the glucose concentration using a conventional method. The proposed wireless biosensor system was also applied to monitor fish stress responses in real time through different stressors and to obtain some precise data that reflect real fish stress responses.
引用
收藏
页数:11
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