Prevalence and Molecular Analyses of Hemotrophic Mycoplasma spp. (Hemoplasmas) Detected in Sika Deer (Cervus nippon yesoensis) in Japan

被引:8
|
作者
Tagawa, Michihito [1 ,2 ]
Matsumoto, Kotaro [1 ]
Yokoyama, Naoaki [3 ]
Inokuma, Hisashi [1 ]
机构
[1] Obihiro Univ Agr & Vet Med, Dept Clin Vet Med, Obihiro, Hokkaido 0808555, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
[3] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
来源
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2014年 / 76卷 / 03期
基金
日本学术振兴会;
关键词
Cervus nippon yesoensis; hemoplasma; Japan; phylogeny; RNA GENE; IDENTIFICATION; OVIS; PCR;
D O I
10.1292/jvms.13-0486
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for 'Candidatus Mycoplasma haemocervae' and 'Candidatus M. erythrocervae', respectively. Sequencing and phylogenetic trees of those three genes indicate that the Candidatus M. haemocervae' and Candidatus M. erythrocervae' detected in Japanese deer are potentially different species from the cervine hemoplasma found in deer from America and Brazil.
引用
收藏
页码:401 / 407
页数:7
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