Cytotoxic Constituents from Vietnamese Marine Sponge Haliclona oculata (Linnaeus, 1759)

Using various chromatography methods, five steroids and two brominated lipids (1-7), 3 beta-hydroxy-5-cholenic acid methyl ester, stigmasta-5,24(28)-dien-3 beta,29-diol, 24-methylenecholest-4-en-3 beta,6 beta-diol, cholest-5-en-3 beta,7 beta-diol, 24-vinylcholest-5-en-3 beta,24-diol, xestospongiene A, and xestospongiene J, respectively, were isolated from the methanol extract of Vietnamese marine sponge Haliclona oculata (Linnaeus, 1759). Their structures were elucidated by 1D-, 2D-NMR, and ESI-MS spectra and in comparison with those reported in the literature. Compounds 1-4, 6, and 7 were reported from Chalinidae family for the first time. In addition, all compounds were evaluated for eight human cancer cell lines as HepG-2, KB, LU-1, MCF-7, LNCaP, HL-60, SK-Mel2, and PC-3. As shown in results, compound 2 showed significant cytotoxic activities on KB and LU-1 cancer cell lines with the IC50 values ranging from 8.74 to 9.32 mu M.