ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Marechal)

被引：13

作者：

Bhadkaria, Amita ^{[1
]}

Gupta, Neha ^{[2
]}

Narvekar, Dakshita Tanaji ^{[1
]}

Bhadkariya, Rajni ^{[1
]}

Saral, Anamika ^{[1
]}

Srivastava, Nidhi ^{[3
]}

Koul, Kirtee Kumar ^{[1
]}

Bhagyawant, Sameer Suresh ^{[1
]}

机构：

[1] Jiwaji Univ, Sch Studies Biotechnol, Gwalior, Madhya Pradesh, India

[2] Rajmata Vijayaraje Scindia Krishi Vishwavidalaya, Plant Mol Biol & Biotechnol Dept, Gwalior, Madhya Pradesh, India

[3] Banasthali Vidyapeeth, Dept Biosci & Biotechnol, Banasthali, Rajasthan, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2020年 / 30卷

关键词：

Moth bean; Underutilised legume; ISSR; Vigna species; Geographic distance;

D O I：

10.1016/j.bcab.2020.101827

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Moth bean (Vigna aconitifolia (Jacq.) Marechal) is considered as one of the underutilised food legume vis-avis the Indian sub-continent. The seeds of this legume are a rich source of soluble proteins for nutrition. PCR-based marker characterizations for assessing genetic diversity are almost negligible in this crop. Present investigation, there fore, employed inter simple sequence repeat (ISSR) markers to assess the genetic diversity amongst 25 accessions. The di and penta nucleotide repeat markers showed higher polymorphism on amplification than those observed using trinucleotide markers. ISSR marker UBC-811 detected 36.15% polymorphism while UBC-879 detected 61.03% with an average of 9.7% on each primer. Based on dendrogram analysis, a logical similarity be tween molecular data and geographical origin was corroborated. The accessions cultivated in west ern coast of India were closely related than north-western and arid regions of India. The accessions like Maru, Jadiya and Jawala can be recommended as parents for further breeding pro grammes to procure additional variation complexes in moth bean genetic stocks.

引用

页数：6

共 26 条

[11]

Gautam A. K., 2016, AGROTECHNOLOGY, V5, P152, DOI [10.4172/2168 -9881. 1000152 ., DOI 10.4172/2168-9881.1000152]

[12] Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence [J].

Gupta, Neha ;

Shrivastava, Nidhi ;

Singh, Pramod Kumar ;

Bhagyawant, Sameer S. .

BIOCHEMISTRY RESEARCH INTERNATIONAL, 2016, 2016

[13] Illumina sequencing of 16S rRNA tag shows disparity in rhizobial and non-rhizobial diversity associated with root nodules of mung bean (Vigrta radiata L.) growing in different habitats in Pakistan [J].

Hakim, Sughra ;

Mirza, Babur S. ;

Imran, Asma ;

Zaheer, Ahmad ;

Yasmin, Sumera ;

Mubeen, Fathia ;

Mclean, Joan E. ;

Mirza, M. Sajjad .

MICROBIOLOGICAL RESEARCH, 2020, 231

[14]

Khadke S. G., 2011, BREEDING PULSE CROPS, P34

[15] DNA isolation from single or half seeds suitable for random amplified polymorphic DNA analyses [J].

Krishna, TG ;

Jawali, N .

ANALYTICAL BIOCHEMISTRY, 1997, 250 (01) :125-127

[16] Seed protein content and its relationships with agronomic traits in pigeonpea is controlled by both main and epistatic effects QTLs [J].

Obala, Jimmy ;

Saxena, Rachit K. ;

Singh, Vikas K. ;

Kale, Sandip M. ;

Garg, Vanika ;

Kumar, C. V. Sameer ;

Saxena, K. B. ;

Tongoona, Pangirayi ;

Sibiya, Julia ;

Varshney, Rajeev K. .

SCIENTIFIC REPORTS, 2020, 10 (01)

[17] Relationship of Phosphorus Fractions with Soil Properties in Mothbean Growing Acid Soils of North Western Indian Himalayas [J].

Shah, Tajamul Islam ;

Rai, A. P. ;

Aziz, M. .

COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 2019, 50 (09) :1192-1198

[18]

Singh P. K., 2014, American Journal of Plant Sciences, V5, P676

[19]

Tomooka N., 2002, The Asian Vigna: genus Vigna subgenus Ceratotropis genetic resources

[20]

Upadhyaya Hari D., 2006, Plant Genetic Resources Characterization and Utilization, V4, P25, DOI 10.1079/PGR2006107

← 1 2 3 →