Analysis of nitrate in biological fluids by capillary electrophoresis

被引：61

作者：

Friedberg, MA ^{[1
]}

Hinsdale, ME ^{[1
]}

Shihabi, ZK ^{[1
]}

机构：

[1] WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT PATHOL,WINSTON SALEM,NC 27157

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 781卷 / 1-2期

关键词：

nitrate;

D O I：

10.1016/S0021-9673(97)00583-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitrite and nitrate represent the products of the final pathway of nitric oxide metabolism. These two ions were analyzed by capillary electrophoresis (CE) in serum, cerebrospinal fluid, urine and tissue homogenates by mixing the sample with acetonitrile containing NaBr as an internal standard, followed by centrifugation. The supernatant was injected hydrodynamically on a capillary 50 cm X 75 mu m (I.D.) and electrophoresed at 6 kV (reversed polarity) in 1.4% sodium chloride in phosphate buffer for 13 min with detection at 214 nm. In addition to removal of the proteins, acetonitrile caused sample stacking. Urinary nitrate analysis by CE was compared to that by the enzymatic Aspergillus nitrate reductase method, with a correlation coefficient of 0.96. (C) 1997 Elsevier Science B.V.

引用

页码：491 / 496

页数：6

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