Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)

被引：45

作者：

Waldschmidt, AM

Salomao, TMF

deBarros, EG

Campos, LDO

机构：

[1] Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG

来源：

BRAZILIAN JOURNAL OF GENETICS | 1997年 / 20卷 / 03期

关键词：

D O I：

10.1590/S0100-84551997000300011

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvae.

引用

页码：421 / 423

页数：3

共 4 条

[1]

Doyle J. J., 1990, Focus, V12, P13, DOI DOI 10.2307/2419362

[2] PATTERNS OF INHERITANCE WITH RAPD MOLECULAR MARKERS REVEAL NOVEL TYPES OF POLYMORPHISM IN THE HONEY-BEE [J].