Photoswitchable Phase Separation and Oligonucleotide Trafficking in DNA Coacervate Microdroplets

被引：131

作者：

Martin, Nicolas ^{[1
,2
,3
]}

Tian, Liangfei ^{[2
,3
,4
]}

Spencer, Dan ^{[2
,3
]}

Coutable-Pennarun, Angelique ^{[4
,5
]}

Anderson, J. L. Ross ^{[4
,5
]}

Mann, Stephen ^{[2
,3
,4
]}

机构：

[1] Univ Bordeaux, Ctr Rech Paul Pascal, CNRS, UMR5031, 115 Ave Dr Albert Schweitzer, F-33600 Pessac, France

[2] Univ Bristol, Ctr Protolife Res, Bristol BS8 1TS, Avon, England

[3] Univ Bristol, Ctr Organized Matter Chem, Sch Chem, Bristol BS8 1TS, Avon, England

[4] Univ Bristol, BrisSynBio Synthet Biol Res Ctr, Life Sci Bldg,Tyndall Ave, Bristol BS8 1TQ, Avon, England

[5] Univ Bristol, Sch Biochem, Bristol BS8 1TD, Avon, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2019年 / 58卷 / 41期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

azobenzene; coacervates; DNA; photochromism; protocells; COMPLEX COACERVATION; PHOTOCONTROL; DROPLETS; CONFORMATION; PROTEINS; BEHAVIOR;

D O I：

10.1002/anie.201909228

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Coacervate microdroplets produced by liquid-liquid phase separation have been used as synthetic protocells that mimic the dynamical organization of membrane-free organelles in living systems. Achieving spatiotemporal control over droplet condensation and disassembly remains challenging. Herein, we describe the formation and photoswitchable behavior of light-responsive coacervate droplets prepared from mixtures of double-stranded DNA and an azobenzene cation. The droplets disassemble and reassemble under UV and blue light, respectively, due to azobenzene trans/cis photoisomerisation. Sequestration and release of captured oligonucleotides follow the dynamics of phase separation such that light-activated transfer, mixing, hybridization, and trafficking of the oligonucleotides can be controlled in binary populations of the droplets. Our results open perspectives for the spatiotemporal control of DNA coacervates and provide a step towards the dynamic regulation of synthetic protocells.

引用

页码：14594 / 14598

页数：5

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