Flies without centrioles

被引:540
作者
Basto, Renata
Lau, Joyce
Vinogradova, Tatiana
Gardiol, Alejandra
Woods, C. Geoffrey
Khodjakov, Alexey
Raff, Jordan W.
机构
[1] Gurdon Inst, Cambridge CB2 1QN, England
[2] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA
[3] SUNY Albany, Dept Biomed Sci, Albany, NY 12222 USA
[4] Cambridge Inst Med Res, Dept Med Genet, Cambridge CB2 2XY, England
基金
英国惠康基金;
关键词
DROSOPHILA ASYMMETRIC DIVISIONS; CELL-CYCLE PROGRESSION; C-ELEGANS; CENTROSOME DUPLICATION; D-TACC; CORTICAL POLARITY; PRIMARY CILIUM; PROTEIN SPD-2; VERTEBRATES; NEUROBLASTS;
D O I
10.1016/j.cell.2006.05.025
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Centrioles and centrosomes have an important role in animal cell organization, but it is uncertain to what extent they are essential for animal development. The Drosophila protein DSas-4 is related to the human microcephaly protein CenpJ and the C. elegans centriolar protein Sas-4. We show that DSas-4 is essential for centriole replication in flies. DSas-4 mutants start to lose centrioles during embryonic development, and, by third-instar larval stages, no centrioles or centrosomes are detectable. Mitotic spindle assembly is slow in mutant cells, and similar to 30% of the asymmetric divisions of larval neuroblasts are abnormal. Nevertheless, mutant flies develop with near normal timing into morphologically normal adults. These flies, however, have no cilia or flagella and die shortly after birth because their sensory neurons lack cilia. Thus, centrioles are essential for the formation of centrosomes, cilia, and flagella, but, remarkably, they are not essential for most aspects of Drosophila development.
引用
收藏
页码:1375 / 1386
页数:12
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