Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells

被引：135

作者：

Fernández-Vizarra, E ^{[1
]}

López-Pérez, MJ ^{[1
]}

Enriquez, JA ^{[1
]}

机构：

[1] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, Zaragoza 50013, Spain

来源：

METHODS | 2002年 / 26卷 / 04期

关键词：

D O I：

10.1016/S1046-2023(02)00034-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes a quick basic method adapted for the purification of mammalian mitochondria from different sources. The organelles obtained using this protocol are suitable for the investigation of biogenetic activities such as enzyme activity, mtDNA, mtRNA, mitochondrial protein synthesis, and mitochondrial tRNA aminoacylation. In addition, these mitochondria are capable of efficient protein import and the investigation of mtDNA/protein interactions by DNA footprinting is also possible. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：292 / 297

页数：6

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