Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum

被引:9
|
作者
Gasparotto, Elainy Patricia Lino
Abrao, Sorrailla Carolina Carrard
Inagaki, Suely Yaeko
Tessmann, Dauri Jose
Kemmelmeier, Carlos
Tessmann, Ione Parra Barbosa [1 ]
机构
[1] Univ Estadual Maringa, Dept Bioquim, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Agron, BR-87020900 Maringa, Parana, Brazil
关键词
galactose oxidase; Fusarium graminearum; enzyme;
D O I
10.1590/S1516-89132006000500004
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer. isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.
引用
收藏
页码:557 / 564
页数:8
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