Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress

被引：65

作者：

Kocheva, K

Lambrev, P

Georgiev, G

Goltsev, V

Karabaliev, M

机构：

[1] Trakia Univ, Fac Med, Dept Phys & Biophys, Stara Zagora 6003, Bulgaria

[2] Bulgarian Acad Sci, Inst Plant Physiol, BU-1113 Sofia, Bulgaria

[3] Bulgarian Acad Sci, Inst Biophys, BU-1113 Sofia, Bulgaria

[4] Univ Sofia, Fac Biol, Dept Biophys & Radiobiol, Sofia 1164, Bulgaria

来源：

BIOELECTROCHEMISTRY | 2004年 / 63卷 / 1-2期

关键词：

chlorophyll fluorescence; PSII; electrolyte leakage; barley; PEG; 8000;

D O I：

10.1016/j.bioelechem.2003.09.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two physiological tests for screening drought tolerance of barley (Hordeum vulgare, L.) plants are compared in this work. Water deficit is induced by treating the plants' roots with polyethylene glycol (PEG 8000). The relative water content (RWC) of the plants is used as a measure of the water status. Conductometrically determined electrolyte leakage from the leaf tissue demonstrates the membrane injury caused by dehydration. It is shown that the injury index increases with the decrease of the RWC of the leaves. The F-v/F-m. ratio is employed to assess changes in the primary photochemical reactions of the photosynthetic apparatus after dehydration. The results suggest that PSII is weakly affected by the imposed osmotic stress. The fluorescence behaviour of the examined cultivars is related to their RWC. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：121 / 124

页数：4

共 21 条

[1] Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants [J].