Influence of flow patterns on chromatographic efficiency in centrifugal partition chromatography

被引：40

作者：

Marchal, L

Foucault, A ^{[1
]}

Patissier, G

Rosant, JM

Legrand, J

机构：

[1] IFREMER, Lab Biochim & Mol Marine, Nantes, France

[2] IUT Nantes, Lab Mecan, Nantes, France

[3] Ecole Cent Nantes, Lab Mecan Fluides, CNRS, URM 6598, Nantes, France

[4] Univ Nantes, Lab Genie Procedes, UPRES EA 1152, St Nazaire, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 869卷 / 1-2期

关键词：

centrifugal partition chromatography; efficiency; flow patterns; Coriolis force;

D O I：

10.1016/S0021-9673(99)01184-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Visualization of flow patterns in centrifugal partition chromatography (CPC) was performed with an asynchronous camera and a stroboscope triggered by the CPC rotor, allowing a channel to be selected and observed regardless of rotational speed. Three main types of flow states were noted as a function of rotational speed and flow-rate: jets stuck along channel walls, broken jets and atomization. Our observations emphasize the importance of Coriolis force on flow shape. Chromatographic efficiency was related to the dispersion of the mobile phase in the stationary phase. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：339 / 352

页数：14

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