Biological imaging with coherent Raman scattering microscopy: a tutorial

被引：50

作者：

Alfonso-Garcia, Alba ^{[1
]}

Mittal, Richa ^{[1
]}

Lee, Eun Seong ^{[2
]}

Potma, Eric O. ^{[1
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92697 USA

[2] Korea Res Inst Stand & Sci, Div Convergence Technol, Ctr Nanobio Technol, Taejon 305340, South Korea

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2014年 / 19卷 / 07期

基金：

美国国家科学基金会;

关键词：

Raman spectroscopy; microscopy; biophotonics; imaging; NONLINEAR-OPTICAL MICROSCOPY; HIGH-SPECTRAL-RESOLUTION; LABEL-FREE; IN-VIVO; QUANTITATIVE-ANALYSIS; CARS MICROSCOPY; CHOLESTEROL CRYSTALS; DIRECT VISUALIZATION; HIGH-SENSITIVITY; LIVING CELLS;

D O I：

10.1117/1.JBO.19.7.071407

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.

引用

页数：13

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