Gene silencing of translationally controlled tumor protein (TCTP) by siRNA inhibits cell growth and induces apoptosis of human prostate cancer cells

被引：69

作者：

Gnanasekar, Munirathinam ^{[1
]}

Thirugnanam, Sivasakthivel ^{[1
]}

Zheng, Guoxing ^{[1
]}

Chen, Aoshuang ^{[1
]}

Ramaswamy, Kalyanasundaram ^{[1
]}

机构：

[1] Univ Illinois, Coll Med, Dept Biomed Sci, Rockford, IL 61107 USA

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2009年 / 34卷 / 05期

关键词：

translationally controlled tumor protein; LNCaP; prostate cancer; siRNA; HISTAMINE-RELEASING FACTOR; TRAIL-INDUCED APOPTOSIS; CALCIUM-BINDING; EXPRESSION; DEATH; FORTILIN; LNCAP; IDENTIFICATION; PROLIFERATION; TUMORIGENESIS;

D O I：

10.3892/ijo_00000252

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Translationally controlled tumor protein (TCTP) is a novel anti apoptotic protein which is highly expressed in several cancer cell types including prostate cancer. However, studies investigating the role of TCTP in prostate cancer are scarce. Therefore, in this study we evaluated the effect of small interference RNA (siRNA) based knocking down of TCTP gene in prostate cancer cells. Cell proliferation and apoptosis were evaluated. Our results showed that TCTP is highly expressed in LNCaP cells compared to normal prostate epithelial cells. Transfection with TCTP siRNA specifically and drastically reduced the expression of both mRNA and protein levels of TCTP in LNCaP cells. The decreased expression of TCTP was associated with decreased viability of LNCaP cells. Further analysis of the transfected LNCaP cells showed that they undergo apoptosis via caspase-8 and caspase-3 dependent pathways. Results presented herein suggest a potential therapeutic application for prostate cancer by targeting TCTP gene using an siRNA approach.

引用

页码：1241 / 1246

页数：6

共 41 条

[1] Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: Expression, distribution, and calcium binding activity [J].

Arcuri, F ;

Papa, S ;

Carducci, A ;

Romagnoli, R ;

Liberatori, S ;

Riparbelli, MG ;

Sanchez, JC ;

Tosi, P ;

del Vecchio, MT .

PROSTATE, 2004, 60 (02) :130-140

[2] Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program [J].

Baudet, C ;

Perret, E ;

Delpech, B ;

Kaghad, M ;

Brachet, P ;

Wion, D ;

Caput, D .

CELL DEATH AND DIFFERENTIATION, 1998, 5 (01) :116-125

[3]

BOHM H, 1989, BIOCHEM INT, V19, P277

[4] The translationally controlled tumour protein (TCTP) [J].

Bommer, UA ;

Thiele, BJ .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (03) :379-385

[5]

Bonnet C, 2000, YEAST, V16, P23, DOI 10.1002/(SICI)1097-0061(20000115)16:1<23::AID-YEA503>3.3.CO

[6]

2-1

[7]

BURGESS A, ONCOGENE IN PRESS

[8] Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A [J].

Cans, C ;

Passer, BJ ;

Shalak, V ;

Nancy-Portebois, V ;

Crible, V ;

Amzallag, N ;

Allanic, D ;

Tufino, R ;

Argentini, M ;

Moras, D ;

Fiucci, G ;

Mirande, M ;

Amson, R ;

Telerman, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :13892-13897

[9] A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner [J].

Chen, Sung Ho ;

Wu, Peih-Shan ;

Chou, Chiang-Hung ;

Yan, Yu-Ting ;

Liu, Hsuan ;

Weng, Shih-Yen ;

Yang-Yen, Hsin-Fang .

MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (07) :2525-2532

[10]

FURUYA Y, 1994, CANCER RES, V54, P6167

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