Laminar organization of the mouse dentate gyrus: Insights from BETA2/Neuro D mutant mice

被引:8
作者
Del Turco, D
Gebhardt, C
Burbach, GJ
Pleasure, SJ
Lowenstein, DH
Deller, T
机构
[1] Goethe Univ Frankfurt, Dept Clin Neuroanat, D-60590 Frankfurt, Germany
[2] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA
关键词
neuronal migration; reelin; anterograde tracing; commissural projection; entorhinal projection;
D O I
10.1002/cne.20239
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The dentate gyrus of rodents is characterized by a highly laminar organization: above a compact granule cell layer, commissural/associational (C/A) fibers terminate on proximal granule cell dendrites and entorhinal fibers terminate on distal granule cell dendrites in a nonoverlapping manner. To gain insights into mechanisms that underlie the formation of this laminar structure, we studied mice deficient for BETA2/NeuroD, a basic helix-loop-helix transcription factor essential for granule cell differentiation. Anterograde tracing was used to label C/A and entorhinal fibers and combined with confocal double immunofluorescence for calbindin, calretinin, parvalbumin, and reelin to visualize putative target cells. The dentate gyrus of mutant mice contained only few granule cells, which formed a cap-like structure adjacent to area CA3. Despite the severe hypoplasia of the dentate gyrus, the remaining BETA2/NeuroD-deficient granule cells expressed mature markers, extended dendrites into the molecular layer, and extended mossy fibers into area CA3. Entorhinal and C/A fibers terminated in a nonoverlapping manner in the dendritic field overlying the rudiment. Entorhinal fibers terminated in the outermost portion of the dentate gyrus where they surrounded reelin-positive Cajal-Retzius cells, and C/A fibers terminated above and within the dentate rudiment. The laminar termination of C/A fibers was closest to normal in zones of the rudiment in which granule cells were densely packed. These data indicate that granule cells are able to differentiate in the absence of BETA2/NeuroD and suggest that the signals underlying the laminar anatomy of the dentate gyrus are present in the absence of most target cells. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码:81 / 95
页数:15
相关论文
共 49 条
[1]   MIGRATION AND DISTRIBUTION OF 2 POPULATIONS OF HIPPOCAMPAL GRANULE CELL PRECURSORS DURING THE PERINATAL AND POSTNATAL PERIODS [J].
ALTMAN, J ;
BAYER, SA .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 301 (03) :365-381
[2]   MOSAIC ORGANIZATION OF THE HIPPOCAMPAL NEUROEPITHELIUM AND THE MULTIPLE GERMINAL SOURCES OF DENTATE GRANULE CELLS [J].
ALTMAN, J ;
BAYER, SA .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 301 (03) :325-342
[3]  
Amaral David G., 1995, P443
[4]   CALCIUM-BINDING PROTEINS IN THE NERVOUS-SYSTEM [J].
BAIMBRIDGE, KG ;
CELIO, MR ;
ROGERS, JH .
TRENDS IN NEUROSCIENCES, 1992, 15 (08) :303-308
[5]   Development of commissural connections in the hippocampus of reeler mice:: Evidence of an inhibitory influence of Cajal-Retzius cells [J].
Borrell, V ;
Ruiz, M ;
Del Río, JA ;
Soriano, E .
EXPERIMENTAL NEUROLOGY, 1999, 156 (02) :268-282
[6]   Transient calretinin expression defines early postmitotic step of neuronal differentiation in adult hippocampal neurogenesis of mice [J].
Brandt, MD ;
Jessberger, S ;
Steiner, B ;
Kronenberg, G ;
Reuter, K ;
Bick-Sander, A ;
von der Behrens, W ;
Kempermann, G .
MOLECULAR AND CELLULAR NEUROSCIENCE, 2003, 24 (03) :603-613
[7]   The repulsive guidance molecule RGMa is involved in the formation of afferent connections in the dentate gyrus [J].
Brinks, H ;
Conrad, S ;
Vogt, J ;
Oldekamp, J ;
Sierra, A ;
Deitinghoff, L ;
Bechmann, I ;
Alvarez-Bolado, G ;
Heimrich, B ;
Monnier, PP ;
Mueller, BK ;
Skutella, T .
JOURNAL OF NEUROSCIENCE, 2004, 24 (15) :3862-3869
[8]   Hippocampal Cajal-Retzius cells project to the entorhinal cortex:: retrograde tracing and intracellular labelling studies [J].
Ceranik, K ;
Deng, JB ;
Heimrich, B ;
Lübke, J ;
Zhao, ST ;
Förster, E ;
Frotscher, M .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (12) :4278-4290
[9]  
Ceranik K, 2000, ANN NY ACAD SCI, V911, P43
[10]   Stereological estimates of total neuron numbers in the hippocampus of adult reeler mutant mice: Evidence for an increased survival of Cajal-Retzius cells [J].
Coulin, C ;
Drakew, A ;
Frotscher, M ;
Deller, T .
JOURNAL OF COMPARATIVE NEUROLOGY, 2001, 439 (01) :19-31