Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

被引：15

作者：

Golovynska, Iuliia ^{[1
]}

Beregova, Tatiana V. ^{[2
]}

Falalyeyeva, Tatiana M. ^{[2
]}

Stepanova, Ludmila I. ^{[2
]}

Golovynskyi, Sergii ^{[1
]}

Qu, Junle ^{[1
]}

Ohulchanskyy, Tymish Y. ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China

[2] Taras Shevchenko Natl Univ Kyiv, Inst Biol & Med, UA-01601 Kiev, Ukraine

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

中国国家自然科学基金;

关键词：

MUCOSAL BLOOD-FLOW; L-GLUTAMIC ACID; ACETYLCHOLINE-RECEPTORS; GLUTAMATERGIC NEURONS; EXPRESSION; CELLS; RESPONSES; SUBTYPES; SUBUNIT; SYSTEM;

D O I：

10.1038/s41598-018-25753-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the present study, we clarified the types of stomach cells that express the NMDARs that are involved in GAS regulation. The pharmacological method of isolated stomach perfusion by Ghosh and Shild combined with direct mapping of NMDARs by fluorescence microscopy in the rat stomach was employed. By immunofluorescence labeling with an anti-NMDA-NR1 antibody, NMDARs were found to be highly expressed in nerve cells of the submucosal and myenteric plexuses in the stomach. The exact localization of the NMDARs relevant to GAS and its mechanism of action were determined by stimulating different receptors of neuronal and stomach cells using specific secretagogues for NMDA and by selectively blocking those receptors. NMDARs relevant to GAS stimulation are mainly localized in cholinergic interneurons; however, all of the nerve cells of the submucosal ganglia are involved in the stimulating process. In addition, the NMDARs in parietal cells are involved in gastric acid inhibition via influencing H-2-histamine receptors.

引用

页数：11

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[1] Cholinergically stimulated gastric acid secretion is mediated by M3 and M5 but not M1 muscarinic acetylcholine receptors in mice [J].