In Silico Characterization of Toxin-Antitoxin Systems in Campylobacter Isolates Recovered from Food Sources and Sporadic Human Illness

被引:1
|
作者
Wadie, Bishoy [1 ]
Abdel-Fattah, Mohamed A. [2 ]
Yousef, Alshymaa [1 ]
Mouftah, Shaimaa F. [1 ]
Elhadidy, Mohamed [1 ,3 ]
Salem, Tamer Z. [1 ,4 ]
机构
[1] Univ Sci & Technol, Zewail City Sci & Technol, Biomed Sci Program, 6th October City, Giza 12578, Egypt
[2] Ain Shams Univ, Fac Agr, Dept Agr Biotechnol, Cairo 11566, Egypt
[3] Mansoura Univ, Fac Vet Med, Dept Bacteriol Mycol & Immunol, Mansoura 35516, Egypt
[4] ARC, AGERI, Dept Microbial Genet, Giza 12619, Egypt
关键词
toxin; antitoxin; Campylobacter; MLST; synteny; in silico; genome; domain; MEDIATED CELL-DEATH; FUNCTIONAL-ANALYSIS; CLONAL COMPLEX; MESSENGER-RNA; IDENTIFICATION; JEJUNI; COLI; GENE; MECHANISM; SEARCH;
D O I
10.3390/genes12010072
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Campylobacter spp. represents the most common cause of gastroenteritis worldwide with the potential to cause serious sequelae. The ability of Campylobacter to survive stressful environmental conditions has been directly linked with food-borne illness. Toxin-antitoxin (TA) modules play an important role as defense systems against antimicrobial agents and are considered an invaluable strategy harnessed by bacterial pathogens to survive in stressful environments. Although TA modules have been extensively studied in model organisms such as Escherichia coli K12, the TA landscape in Campylobacter remains largely unexplored. Therefore, in this study, a comprehensive in silico screen of 111 Campylobacter (90 C. jejuni and 21 C. coli) isolates recovered from different food and clinical sources was performed. We identified 10 type II TA systems belonging to four TA families predicted in Campylobacter genomes. Furthermore, there was a significant association between the clonal population structure and distribution of TA modules; more specifically, most (12/13) of the Campylobacter isolates belonging to ST-21 isolates possess HicB-HicA TA modules. Finally, we observed a high degree of shared synteny among isolates bearing certain TA systems or even coexisting pairs of TA systems. Collectively, these findings provide useful insights about the distribution of TA modules in a heterogeneous pool of Campylobacter isolates from different sources, thus developing a better understanding regarding the mechanisms by which these pathogens survive stressful environmental conditions, which will further aid in the future designing of more targeted antimicrobials.
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页码:1 / 16
页数:16
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