A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator

被引：50

作者：

Uchiyama, Seiichi ^{[1
]}

Tsuji, Toshikazu ^{[2
]}

Kawamoto, Kyoko ^{[1
]}

Okano, Kentaro ^{[3
,4
]}

Fukatsu, Eiko ^{[1
]}

Noro, Takahiro ^{[3
]}

Ikado, Kumiko ^{[2
]}

Yamada, Sayuri ^{[2
]}

Shibata, Yuka ^{[1
]}

Hayashi, Teruyuki ^{[5
,6
]}

Inada, Noriko ^{[5
]}

Kato, Masaru ^{[1
]}

Koizumi, Hideki ^{[2
]}

Tokuyama, Hidetoshi ^{[3
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

[2] KIRIN Co Ltd, Cent Labs Key Technol, Kanazawa Ku, 1-13-5 Fukuura, Yokohama, Kanagawa 2360004, Japan

[3] Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi 9808578, Japan

[4] Kobe Univ, Dept Chem Sci & Engn, Kobe, Hyogo 6578501, Japan

[5] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300192, Japan

[6] Koshien Univ, Dept Nutr, 10-1 Momijigaoka, Takarazuka, Hyogo 6650006, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 19期

关键词：

fluorescence spectroscopy; fluorescent probes; imaging agents; nanoparticles; sensors; INTRACELLULAR TEMPERATURE-MEASUREMENTS; POLYMERIC THERMOMETERS; MAMMALIAN-CELLS; ENERGY-TRANSFER; AQUEOUS-MEDIA; NANOPARTICLES; LUMINESCENT; DECOMPOSITION; PROTEINS; DELIVERY;

D O I：

10.1002/anie.201801495

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A cationic fluorescent nanogel thermometer based on thermo-responsive N-isopropylacrylamide and environment-sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02-0.84 degrees C in the range 20-40 degrees C), and remarkable non-cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells.

引用

页码：5413 / 5417

页数：5

共 46 条

[1] Mitochondria-targeted fluorescent thermometer monitors intracellular temperature gradient [J].

Arai, Satoshi ;

Suzuki, Madoka ;

Park, Sung-Jin ;

Yoo, Jung Sun ;

Wang, Lu ;

Kang, Nam-Young ;

Ha, Hyung-Ho ;

Chang, Young-Tae .

CHEMICAL COMMUNICATIONS, 2015, 51 (38) :8044-8047

[2] Micro/Nanoscale Thermometry for Cellular Thermal Sensing [J].

Bai, Tingting ;

Gu, Ning .

SMALL, 2016, 12 (34) :4590-4610

[3]

Baker SN, 2005, ACS SYM SER, V902, P171

[4] SYNTHETIC GENE-TRANSFER VECTORS [J].

BEHR, JP .

ACCOUNTS OF CHEMICAL RESEARCH, 1993, 26 (05) :274-278

[5]

Brites CDS, 2016, HBK PHYS CHEM RARE, V49, P339, DOI 10.1016/bs.hpcre.2016.03.005

[6] Thermometry at the nanoscale [J].

Brites, Carlos D. S. ;

Lima, Patricia P. ;

Silva, Nuno J. O. ;

Millan, Angel ;

Amaral, Vitor S. ;

Palacio, Fernando ;

Carlos, Luis D. .

NANOSCALE, 2012, 4 (16) :4799-4829

[7]

Carlos L. D., 2016, Thermometry at the Nanoscale: Techniques and Selected Applications

[8] A PNIPAM-based fluorescent nanothermometer with ratiometric readout [J].

Chen, Chun-Yen ;

Chen, Chao-Tsen .

CHEMICAL COMMUNICATIONS, 2011, 47 (03) :994-996

[9] Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications [J].

Dalby, B ;

Cates, S ;

Harris, A ;

Ohki, EC ;

Tilkins, ML ;

Price, PJ ;

Ciccarone, VC .

METHODS, 2004, 33 (02) :95-103

[10] Mapping Intracellular Temperature Using Green Fluorescent Protein [J].

Donner, Jon S. ;

Thompson, Sebastian A. ;

Kreuzer, Mark P. ;

Baffou, Guillaume ;

Quidant, Romain .

NANO LETTERS, 2012, 12 (04) :2107-2111

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