Application of ΦSP-1 and ΦSP-3 as a therapeutic strategy against Salmonella Enteritidis infection using Caenorhabditis elegans as model organism

被引：11

作者：

Augustine, Jeena ^{[1
]}

Gopalakrishnan, Mridula V. ^{[1
]}

Bhat, Sarita G. ^{[1
]}

机构：

[1] Cochin Univ Sci & Technol, Dept Biotechnol, Cochin 22, Kerala, India

来源：

FEMS MICROBIOLOGY LETTERS | 2014年 / 356卷 / 01期

关键词：

Enteritidis; lytic phage; biocontrol agent; antibiotic resistance; LYTIC PHAGE; HOST; RESISTANCE; PATHOGENESIS; COIMBATORE; PREVALENCE; COCKTAIL; MARKETS;

D O I：

10.1111/1574-6968.12493

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The potential of Salmonella-specific phages Phi SP-1 and Phi SP-3 as biocontrol agents was studied in vitro, employing host cell lysis test and in vivo, using Caenorhabditis elegans as a model organism. For in vivo testing, stage 4 C. elegans larvae were experimentally infected with the pathogen Salmonella. Worm mortality was scored for 10 days. TD50 (the time required for 50% of the nematodes to die) of infected worms in the presence of bacteriophages was comparable to uninfected worms, and the two phages provided an increased protection than each one. This study in addition demonstrated the simplicity, elegance, and the cost effectiveness of the C. elegans model for in vivo validation.

引用

页码：113 / 117

页数：5