Controlled charged amino acids of Ti-binding peptide for surfactant-free selective adsorption

被引：8
作者：
Fukuta, Megumi [1 ,3 ]
Zheng, Bin [2 ,3 ]
Uenuma, Mutsunori [2 ,3 ]
Okamoto, Naofumi [2 ]
Uraoka, Yukiharu [2 ,3 ]
Yamashita, Ichiro [2 ,3 ]
Watanabe, Heiji [1 ,3 ]
机构：
[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan
[2] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan
[3] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020075, Japan
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2014年 / 118卷
基金：
日本科学技术振兴机构;
关键词：
Titanium-binding peptide; Ferritin; Adsorption mechanism; Selective adsorption; SINGLE-ELECTRON TRANSISTORS; PROTEIN SUPRAMOLECULE; METAL NANOPARTICLES; INORGANIC MATERIALS; OPTICAL-PROPERTIES; LASER-ABLATION; FERRITIN; GOLD; SPECIFICITY; TITANIUM;
D O I：
10.1016/j.colsurfb.2014.03.024
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The adsorption mechanism of titanium-binding peptide (TBP) on metal oxide substrates was investigated by evaluating the adsorption behavior of ferritins with various alanine-substituted TBPs. Results revealed that (a) a positively charged amino acid, lysine (K) or arginine (R), in TBP can anchor ferritin to negative zeta-potential substrates, (b) the adsorption force of K is stronger than R, and (c) local electrostatic interactions and flexibility of TBP directly affect adsorption. Based on these findings, selective ferritin adsorption on SiO2 with TiOx patterned surfaces in a surfactant-free condition was demonstrated. Alanine-substituted TBP with one positively charged amino acid (K) and one negatively charged amino acid (D), achieved ferritin-selective adsorption without a surfactant. The importance of controlled electrostatic forces between TBP and a substrate for selective adsorption without a surfactant was clearly demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：25 / 30
页数：6
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