Controlled charged amino acids of Ti-binding peptide for surfactant-free selective adsorption

被引：8

作者：

Fukuta, Megumi ^{[1
,3
]}

Zheng, Bin ^{[2
,3
]}

Uenuma, Mutsunori ^{[2
,3
]}

Okamoto, Naofumi ^{[2
]}

Uraoka, Yukiharu ^{[2
,3
]}

Yamashita, Ichiro ^{[2
,3
]}

Watanabe, Heiji ^{[1
,3
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan

[3] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2014年 / 118卷

基金：

日本科学技术振兴机构;

关键词：

Titanium-binding peptide; Ferritin; Adsorption mechanism; Selective adsorption; SINGLE-ELECTRON TRANSISTORS; PROTEIN SUPRAMOLECULE; METAL NANOPARTICLES; INORGANIC MATERIALS; OPTICAL-PROPERTIES; LASER-ABLATION; FERRITIN; GOLD; SPECIFICITY; TITANIUM;

D O I：

10.1016/j.colsurfb.2014.03.024

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The adsorption mechanism of titanium-binding peptide (TBP) on metal oxide substrates was investigated by evaluating the adsorption behavior of ferritins with various alanine-substituted TBPs. Results revealed that (a) a positively charged amino acid, lysine (K) or arginine (R), in TBP can anchor ferritin to negative zeta-potential substrates, (b) the adsorption force of K is stronger than R, and (c) local electrostatic interactions and flexibility of TBP directly affect adsorption. Based on these findings, selective ferritin adsorption on SiO2 with TiOx patterned surfaces in a surfactant-free condition was demonstrated. Alanine-substituted TBP with one positively charged amino acid (K) and one negatively charged amino acid (D), achieved ferritin-selective adsorption without a surfactant. The importance of controlled electrostatic forces between TBP and a substrate for selective adsorption without a surfactant was clearly demonstrated. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：25 / 30

页数：6

共 55 条

[1] THE USE OF TWEEN-20 AS A BLOCKING-AGENT IN THE IMMUNOLOGICAL DETECTION OF PROTEINS TRANSFERRED TO NITROCELLULOSE MEMBRANES [J].

BATTEIGER, B ;

NEWHALL, WJ ;

JONES, RB .

JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 55 (03) :297-307

[2] Metal nanoparticles generated by laser ablation [J].

Becker, MF ;

Brock, JR ;

Cai, H ;

Henneke, DE ;

Keto, JW ;

Lee, JY ;

Nichols, WT ;

Glicksman, HD .

NANOSTRUCTURED MATERIALS, 1998, 10 (05) :853-863

[3]

Bos MA, 2001, ADV COLLOID INTERFAC, V91, P437

[4] PLASMA RESONANCE ENHANCEMENT OF RAMAN-SCATTERING BY PYRIDINE ADSORBED ON SILVER OR GOLD SOL PARTICLES OF SIZE COMPARABLE TO THE EXCITATION WAVELENGTH [J].

CREIGHTON, JA ;

BLATCHFORD, CG ;

ALBRECHT, MG .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS II, 1979, 75 :790-798

[5] Recent advances in the liquid-phase syntheses of inorganic nanoparticles [J].

Cushing, BL ;

Kolesnichenko, VL ;

O'Connor, CJ .

CHEMICAL REVIEWS, 2004, 104 (09) :3893-3946

[6] The Effect of Nanoparticle Shape on the Photocarrier Dynamics and Photovoltaic Device Performance of Poly(3-hexylthiophene):CdSe Nanoparticle Bulk Heterojunction Solar Cells [J].

Dayal, Smita ;

Reese, Matthew O. ;

Ferguson, Andrew J. ;

Ginley, David S. ;

Rumbles, Garry ;

Kopidakis, Nikos .

ADVANCED FUNCTIONAL MATERIALS, 2010, 20 (16) :2629-2635

[7] Nanophase cobalt oxyhydroxide mineral synthesized within the protein cage of ferritin [J].

Douglas, T ;

Stark, VT .

INORGANIC CHEMISTRY, 2000, 39 (08) :1828-1830

[8] Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].

Elghanian, R ;

Storhoff, JJ ;

Mucic, RC ;

Letsinger, RL ;

Mirkin, CA .

SCIENCE, 1997, 277 (5329) :1078-1081

[9]

Feldheim DL, 1998, CHEM SOC REV, V27, P1

[10] FERRITIN - DESIGN AND FORMATION OF AN IRON-STORAGE MOLECULE [J].

FORD, GC ;

HARRISON, PM ;

RICE, DW ;

SMITH, JMA ;

TREFFRY, A ;

WHITE, JL ;

YARIV, J .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1984, 304 (1121) :551-+

← 1 2 3 4 5 6 →