Effect of carboxyl density at the core-shell interface of surface-imprinted magnetic trilayer microspheres on recognition properties of proteins

被引：24

作者：

Li, Xiangjie ^{[1
]}

Zhang, Baoliang ^{[1
]}

Tian, Lei ^{[1
]}

Li, Wei ^{[1
]}

Xin, Tiejun ^{[1
]}

Zhang, Hepeng ^{[1
]}

Zhang, Qiuyu ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Sch Sci, Key Lab Appl Phys & Chem Space, Minist Educ, Xian 710072, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 196卷

基金：

国家高技术研究发展计划(863计划);

关键词：

Surface imprinting; Carboxyl density; Protein recognition; Magnetic microspheres; SILICA NANOPARTICLES; BOVINE HEMOGLOBIN; POLYMER; MONOMER; SENSOR;

D O I：

10.1016/j.snb.2014.01.109

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel protein surface-imprinted magnetic trilayer composite microsphere (Fe3O4@HEA@protein-MIPs) was designed and prepared. The objective of this study was to study the effect of carboxyl density at the core-shell interface on recognition performance. The imprinting process was carried out on the surface of Fe3O4@hydroxyethyl acrylate (Fe3O4@HEA) microsphere modified by maleic anhydride (MAH) in the presence of functional monomer acrylamide (AM) and cross-linker N,N'-methylenebisacrylamide (MBA). The results revealed that the adsorption rate, adsorption capacity and recognition capacity of surface-imprinted magnetic microsphere increased with increasing carboxyl density. Meanwhile, the increase of carboxyl density was conducive to improve the imprinting efficiency of the proteins with high isoelectric point (pI > 7). Furthermore, the effect of carboxyl at the core-shell interface on imprinting efficiency was restricted by the thickness of imprinted layer, and the critical thickness which related to the size of template protein was obtained by experiments. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：265 / 271

页数：7

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