Tamoxifen Improves Glucose Tolerance in a Delivery-, Sex-, and Strain-Dependent Manner in Mice

被引：23

作者：

Ceasrine, Alexis M. ^{[1
]}

Ruiz-Otero, Nelmari ^{[1
]}

Lin, Eugene E. ^{[1
]}

Lumelsky, David N. ^{[1
]}

Boehm, Erica D. ^{[1
]}

Kuruvilla, Rejji ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

ENDOCRINOLOGY | 2019年 / 160卷 / 04期

基金：

美国国家卫生研究院;

关键词：

PANCREATIC BETA-CELLS; ESTROGEN-RECEPTOR; IN-VIVO; ISLETS; ACTIVATION; APOPTOSIS; BINDING; OBESITY;

D O I：

10.1210/en.2018-00985

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Tamoxifen, a selective estrogen-receptor modulator, is widely used in mouse models to temporally control gene expression but is also known to affect body composition. We report that tamoxifen has significant and sustained effects on glucose tolerance, independent of effects on insulin sensitivity, in mice. IP, but not oral, tamoxifen delivery improved glucose tolerance in three inbred mouse strains. The extent and persistence of tamoxifen-induced effects were sex and strain dependent. These findings highlight the need to revise commonly used tamoxifen-based protocols for gene manipulation in mice by including longer chase periods after injection, oral delivery, and the use of tamoxifen-treated littermate controls.

引用

页码：782 / 790

页数：9

共 30 条

[1] Effect of 17β-estradiol on insulin secretion and cytosolic calcium in MIN6 mouse insulinoma cells and human islets of Langerhans [J].

Al-Majed, HT ;

Squires, PE ;

Persaud, SJ ;

Huang, GCC ;

Amiel, S ;

Whitehouse, BJ ;

Jones, PM .

PANCREAS, 2005, 30 (04) :307-313

[2] Loss of Nuclear and Membrane Estrogen Receptor-α Differentially Impairs Insulin Secretion and Action in Male and Female Mice [J].

Allard, Camille ;

Morford, Jamie J. ;

Xu, Beibei ;

Salwen, Benjamin ;

Xu, Weiwei ;

Desmoulins, Lucie ;

Zsombok, Andrea ;

Kim, Jason K. ;

Levin, Ellis R. ;

Mauvais-Jarvis, Franck .

DIABETES, 2019, 68 (03) :490-501

[3] Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts [J].

Andersson, Kristin B. ;

Winer, Lisbeth H. ;

Mork, Halvor K. ;

Molkentin, Jeffery D. ;

Jaisser, Frederic .

TRANSGENIC RESEARCH, 2010, 19 (04) :715-725

[4] Evaluating the glucose tolerance test in mice [J].

Andrikopoulos, Sofianos ;

Blair, Amy R. ;

Deluca, Nadia ;

Fam, Barbara C. ;

Proietto, Joseph .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2008, 295 (06) :E1323-E1332

[5] RATES AND TISSUE SITES OF NON-INSULIN-MEDIATED AND INSULIN-MEDIATED GLUCOSE-UPTAKE IN HUMANS [J].

BARON, AD ;

BRECHTEL, G ;

WALLACE, P ;

EDELMAN, SV .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (06) :E769-E774

[6] An action plan for mouse genomics [J].

Battey, J ;

Jordan, E ;

Cox, D ;

Dove, W .

NATURE GENETICS, 1999, 21 (01) :73-75

[7] Glucose metabolism in vivo in four commonly used inbred mouse strains [J].

Berglund, Eric D. ;

Li, Candice Y. ;

Poffenberger, Greg ;

Ayala, Julio E. ;

Fueger, Patrick T. ;

Willis, Shannon E. ;

Jewell, Marybeth M. ;

Powers, Alvin C. ;

Wasserman, David H. .

DIABETES, 2008, 57 (07) :1790-1799

[8] Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism [J].

Chiang, Po-Min ;

Ling, Jonathan ;

Jeong, Yun Ha ;

Price, Donald L. ;

Aja, Susan M. ;

Wong, Philip C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (37) :16320-16324

[9] Activation of estrogen receptor is crucial for resveratrol-stimulating muscular glucose uptake via both insulin-dependent and -independent pathways [J].

Deng, Jen-Ying ;

Hsieh, Po-Shiuan ;

Huang, Jiung-Pang ;

Lu, Long-Sheng ;

Hung, Li-Man .

DIABETES, 2008, 57 (07) :1814-1823

[10] Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains [J].

Feil, R ;

Wagner, J ;

Metzger, D ;

Chambon, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 237 (03) :752-757

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