Tamoxifen Improves Glucose Tolerance in a Delivery-, Sex-, and Strain-Dependent Manner in Mice

被引：22

作者：

Ceasrine, Alexis M. ^{[1
]}

Ruiz-Otero, Nelmari ^{[1
]}

Lin, Eugene E. ^{[1
]}

Lumelsky, David N. ^{[1
]}

Boehm, Erica D. ^{[1
]}

Kuruvilla, Rejji ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

ENDOCRINOLOGY | 2019年 / 160卷 / 04期

基金：

美国国家卫生研究院;

关键词：

PANCREATIC BETA-CELLS; ESTROGEN-RECEPTOR; IN-VIVO; ISLETS; ACTIVATION; APOPTOSIS; BINDING; OBESITY;

D O I：

10.1210/en.2018-00985

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Tamoxifen, a selective estrogen-receptor modulator, is widely used in mouse models to temporally control gene expression but is also known to affect body composition. We report that tamoxifen has significant and sustained effects on glucose tolerance, independent of effects on insulin sensitivity, in mice. IP, but not oral, tamoxifen delivery improved glucose tolerance in three inbred mouse strains. The extent and persistence of tamoxifen-induced effects were sex and strain dependent. These findings highlight the need to revise commonly used tamoxifen-based protocols for gene manipulation in mice by including longer chase periods after injection, oral delivery, and the use of tamoxifen-treated littermate controls.

引用

页码：782 / 790

页数：9

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