Simultaneous determination of 18 abused opioids and metabolites in human hair using LC-MS/MS and illegal opioids abuse proven by hair analysis

被引:39
作者
Kim, Jihyun [1 ]
Ji, Dajeong [2 ]
Kang, Soyoung [2 ]
Park, Meejung [1 ]
Yang, Wonkyung [1 ]
Kim, Eunmi [1 ]
Choi, Hwakyung [3 ]
Lee, Sooyeun [2 ]
机构
[1] Natl Forens Serv, Seoul 158707, South Korea
[2] Keimyung Univ, Coll Pharm, Taegu 704701, South Korea
[3] Natl Forens Serv, Southern Dist Off, Yangsan 626815, Gyeongsangnam D, South Korea
关键词
Opioids; Drug abuse; Hair analysis; LC-MS/MS; Method validation; MASS-SPECTROMETRY; OPIATES; DRUG; PROGRAM; COCAINE; URINE;
D O I
10.1016/j.jpba.2013.10.041
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Natural and synthetic opioids have efficient analgesic activity but can also be addictive. Thus, the determination of opioids and their metabolites in biological specimens is of interest in clinical and forensic toxicology laboratories. The analysis of drugs in hair provides valuable information on previous chronic drug use and has been successfully applied to the diagnosis of drug abuse, tolerance, compliance and gestational drug exposure. Despite the abuse of prescription opioids along with heroin and other illegal opiates, few studies have been conducted on the simultaneous determination of the broad range of opioids covering those drugs in hair. In the present study, an analytical method for the simultaneous detection in hair of 18 opioids and metabolites considered to have a high abuse risk based on the results of urine drug screening was established and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the purpose of clinical and forensic applications. The drugs and metabolites were extracted from hair using methanol and analyzed using LC-MS/MS. The validation results proved that the method was selective, accurate and precise with acceptable linearity within calibration ranges. No significant variation was observed by different sources of matrices. The limits of detection and the limits of quantification ranged from 0.05 to 0.25 ng/10 mg hair and from 0.05 to 0.5 ng/10 mg hair, respectively. The developed method was successfully applied to 15 hair samples from opioids users. This method will be very useful for monitoring the inappropriate use of opioid drugs. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:99 / 105
页数:7
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