Anti-double stranded DNA antibodies in systemic lupus erythematosus: Detection and clinical relevance of IgM-class antibodies

被引：25

作者：

Bootsma, H ^{[1
]}

Spronk, PE ^{[1
]}

Hummel, EJ ^{[1
]}

deBoer, G ^{[1
]}

terBorg, EJ ^{[1
]}

Limburg, PC ^{[1
]}

Kallenberg, CGM ^{[1
]}

机构：

[1] UNIV GRONINGEN HOSP, DIV CLIN IMMUNOL, NL-9700 RB GRONINGEN, NETHERLANDS

来源：

SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1996年 / 25卷 / 06期

关键词：

systemic lupus erythematosus; anti-dsDNA; immunoglobulin classes; ELISA; Farr assay; Crithidia luciliae;

D O I：

10.3109/03009749609065646

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We determined the discriminative value of the Farr assay in comparison to ELISA and Crithidia luciliae immunofluorescence assay (IFT) for detecting anti-dsDNA antibodies as a diagnostic tool for systemic lupus erythematosus (SLE). Special attention was paid to the diagnostic significance of IgM-class anti-dsDNA. Sera were analyzed from 74 patients with SLE, 257 patients with other auto-immune diseases, and 50 healthy controls. All sera were tested for anti-dsDNA using the IFT (anti-total immunoglobulin conjugate), ELISA (anti-IgG and anti-IgM conjugates), and the I-125 Farr assay. Specificity and sensitivity for a diagnosis of SLE appeared to be highest for the Farr. All SLE sera with IgM-class anti-dsDNA without IgG-class anti-dsDNA as detected by ELISA, were positive when tested by the Farr assay. In contrast, most of the sera with IgM-class anti-dsDNA as detected by ELISA from patients with diseases other than SLE were negative when tested by Farr assay.

引用

页码：352 / 359

页数：8

共 30 条

[1] IMMUNOLOGY OF DNA .3. CRITHIDIA-LUCILIAE, A SIMPLE SUBSTRATE FOR DETERMINATION OF ANTI-DSDNA WITH IMMUNOFLUORESCENCE TECHNIQUE [J].

AARDEN, LA ;

DEGROOT, ER ;

FELTKAMP, TEW .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1975, 254 :505-515

[2]

ARNETT FC, 1987, ARTHRITIS RHEUM, V30, P47

[3] ANTI-NATIVE DNA DETECTION BY THE CRITHIDIA-LUCILIAE METHOD - IMPROVED GUIDE TO THE DIAGNOSIS AND CLINICAL MANAGEMENT OF SYSTEMIC LUPUS-ERYTHEMATOSUS [J].

BALLOU, SP ;

KUSHNER, I .

ARTHRITIS AND RHEUMATISM, 1979, 22 (04) :321-327

[4]

BALLOU SP, 1979, CLIN EXP IMMUNOL, V37, P58

[5] DERIVATION OF THE SLEDAI - A DISEASE-ACTIVITY INDEX FOR LUPUS PATIENTS [J].

BOMBARDIER, C ;

GLADMAN, DD ;

UROWITZ, MB ;

CARON, D ;

CHANG, CH .

ARTHRITIS AND RHEUMATISM, 1992, 35 (06) :630-640

[6] PREVENTION OF RELAPSES IN SYSTEMIC LUPUS-ERYTHEMATOSUS [J].

BOOTSMA, H ;

SPRONK, P ;

DERKSEN, R ;

DEBOER, G ;

WOLTERSDICKE, H ;

HERMANS, J ;

LIMBURG, P ;

GMELIGMEYLING, F ;

KATER, L ;

KALLENBERG, C .

LANCET, 1995, 345 (8965) :1595-1599

[7]

BORGTER EJ, 1990, ARTHRITIS RHEUM, V33, P634

[8] CROSS-REACTIVE BINDING PATTERNS OF MONOCLONAL-ANTIBODIES TO DNA ARE OFTEN CAUSED BY DNA ANTI-DNA IMMUNE-COMPLEXES [J].

BRINKMAN, K ;

TERMAAT, RM ;

DEJONG, J ;

VANDENBRINK, HG ;

BERDEN, JHM ;

SMEENK, RJT .

RESEARCH IN IMMUNOLOGY, 1989, 140 (5-6) :595-612

[9]

BRINKMAN K, 1990, CLIN RHEUMATOL S1, V9, P63

[10]

Daniels TE, 1987, SJOGRENS SYNDROME CL, P193

← 1 2 3 →