Ca2+ sequestration as a determinant of chaos and mixed-mode dynamics in agonist-induced vasomotion

被引:26
作者
Griffith, TM [1 ]
Edwards, DH [1 ]
机构
[1] UNIV WALES COLL MED, DEPT CARDIOL, CARDIOVASC SCI RES GRP, CARDIFF CF4 4XN, S GLAM, WALES
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1997年 / 272卷 / 04期
关键词
sarcoplasmic reticulum; cyclopiazonic acid; thapsigargin; ryanodine; charybdotoxin; ouabain;
D O I
10.1152/ajpheart.1997.272.4.H1696
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have investigated the contribution of smooth muscle Ca2+ stores to chaotic vasomotion in isolated rabbit ear resistance arteries. In preparations constricted by histamine, exposure to cyclopiazonic acid (CPA) and thapsigargin (TSG), which inhibit the Ca2+- adenosinetriphosphatase (ATPase) pump of the sarcoplasmic reticulum, first induced then abolished highly characteristic mixed-mode oscillatory behavior. The fractal dimension of the vasomotion, which reflects the minimum number of contributing dynamic variables, remained between 2 and 4 until the point at which oscillations disappeared completely. By contrast, ryanodine, which attenuates Ca2+-induced Ca2+ release, decreased the fractal dimension of the responses to <2 in a graded concentration-dependent fashion by selectively suppressing a slow subcomponent of the overall rhythmic activity. CPA-associated oscillations were insensitive to ryanodine but could be abolished by verapamil and modulated in an inhibitory or stimulatory fashion by charybdotoxin, which blocks Ca2+-activated K+ channels, and by ouabain, which blocks the Na+-K+-ATPase. We conclude that there is nonlinear cross talk between CPA/TSG-sensitive Ca2+ stores and a membrane oscillator that regulates Ca2+ influx and that the kinetics of Ca2+ uptake by the CPA/TSG-sensitive pool can be distinguished dynamically from the kinetics of Ca2+ release from its ryanodine-sensitive subcomponent.
引用
收藏
页码:H1696 / H1709
页数:14
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