Evaluation of Efficacy of Compound Chinese Medicinal Herbs against Mycoplasma synoviae using different Lab Tests in Mouse and Chicken

The present study was designed to treat the Mycoplasma synovaie (MS) by Chinese herbs extract (CHE). In the current study, prepared drug sensitivity and safety test was carried out by culture medium and on mice model against MS, respectively. The sensitivity test results showed that the sensitivity of MS towards Rheum rhabarbarum and Lysimachia davurica was highest, followed by the Lonicera japonica, Codonopsis pilosula and Radix liquiritiae. Drug safety experiment results showed that LD50 of the compound Chinese medicine preparation was 41438 mg/kg. For chicken pathological model, 50 six-week old chicks were divided into five groups. First group was considered as a negative control group, sec group was considered as a positive control (infected with MS), 3rd group infected with MS and supplemented with CHE @ 2 mL/bird/day, 4th group infected with MS and supplemented with CHE @ 1.5 mL/bird/day and 5th group infected with MS and supplemented with CHE @ 1 mL/bird/day for 3 days, respectively. In chicken pathological model, results of ELISA and culture medium test showed that group 3 had a better recovery response towards MS. The results of ELISA showed that the MS were found in each group at day 3. At 6 and 9 day, 4th and 5th group showed MS sign. At day 12, 3rd and 4th group didn't show any MS sign. At day 15 and 18, no MS signs were observed in all groups. Based on findings, it is concluded that Chinese herbal medicine @ 2.5 mL/bird showed a better resistance towards MS. (C) 2019 Friends Science Publishers