Molecular Cloning and Nucleotide Sequence Analysis of the Newly Identified UL53 Gene of Duck Enteritis Virus

被引：0
作者：
Zhang, Shunchuan [1 ]
Cheng, Anchun [1 ,2 ]
Wang, Mingshu [1 ,2 ]
Xiang, Jun [1 ]
Cheng, Anchun [1 ,2 ]
Wang, Mingshu [1 ,2 ]
机构：
[1] Sichuan Agr Univ, Coll Vet Med, Avian Dis Res Ctr, Yaan, Peoples R China
[2] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Yaan, Peoples R China
来源：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年
基金：
中国国家自然科学基金;
关键词：
Bioinfomatics; Cloning; Nucleotide sequence analysis; DEV; UL53; gene; DUCK VIRAL-ENTERITIS; EVOLUTIONARY TIMESCALE; MOLECULAR PHYLOGENY; PLAGUE; HERPESVIRUS-1; EXPRESSION; SEQUENCE; PROTEIN; GENE; DNA;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Duck enteritis virus (DEV) is an alphaherpesvirinae that causes an acute, contagious, and fatal disease. In this study, the DEV-UL53 gene from Key Laboratory of Animal Disease and Human Health of Sichuan Province was cloned and sequenced. Nucleotide sequence analysis revealed a 1032 bp open reading frame (ORF) theoretically encoding a 343-amino acid polypeptide was homologous to UL53 gene of alphaherpesvirinaes but there was just a predicted 226aa peptide, which could be expressed facilely, by using GENSCAN. The DEV-UL53 gene had a base composition of 270 adenine (26.2%), 206cytosine (20.0%), 232 guanine (22.5%) and 324 thymine (31.4%). Compared with the UL53 gene of other alphaherpesvirinaes, the DEV-UL53 gene had a close evolutionary relationship with members of the Mardivirus genus. Therefore, the phylogenetic tree of UL53 might be used as a basis for DEV classification. In conclusion, all data will provide a basis for further functional analysis of the DEV-UL53 gene.
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页数：6
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