Effect of estrogen on the expression of GnRH and kisspeptin in the hypothalamus of rats during puberty

被引：11
作者：
Cui, Pei [1 ]
Yang, Chen [1 ,2 ,3 ]
Zhang, Kaifa [1 ,2 ,3 ]
Gao, Xiaoxiao [1 ,2 ,3 ]
Luo, Lei [1 ,2 ,3 ]
Tian, Yuan [1 ,3 ]
Song, Min [1 ,3 ]
Liu, Ya [1 ,2 ,3 ]
Zhang, Yunhai [1 ,2 ,3 ]
Li, Yunsheng [1 ,3 ]
Zhang, Xiaorong [1 ,2 ,3 ]
Su, Shiping [1 ,2 ,3 ]
Fang, Fugui [1 ,2 ,3 ]
Ding, Jianping [1 ,2 ,3 ]
机构：
[1] Anhui Agr Univ, Coll Anim Sci & Technol, Dept Anim Sci, Anhui Prov Lab Anim Genet Resources Protect & Bre, Hefei, Anhui, Peoples R China
[2] Anhui Agr Univ, Coll Anim Sci & Technol, Dept Anim Sci, Anhui Prov Lab Local Livestock & Poultry Genet Re, Hefei, Anhui, Peoples R China
[3] Anhui Agr Univ, Coll Anim Sci & Technol, Dept Anim Vet Sci, Hefei, Anhui, Peoples R China
来源：
THERIOGENOLOGY | 2015年 / 84卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Kisspeptin; GnRH; Estrogen; Rat; Puberty; GONADOTROPIN-RELEASING-HORMONE; NEUROKININ B; DEVELOPMENTAL-CHANGES; LUTEINIZING-HORMONE; STEROID REGULATION; SEXUAL-DIMORPHISM; GENE-EXPRESSION; ARCUATE NUCLEUS; PREOPTIC AREA; NEURONS;
D O I：
10.1016/j.theriogenology.2015.08.004
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to assess whether changes in kisspeptin and GnRH levels could be attributed to sex steroids at puberty onset. We used the ovariectomy (OVX) model in rats treated with 17 beta-estradiol (E-2; OVX + E-2), or oil (OVX + oil), and in intact rats treated with E-2 (intact + E-2) or oil only (intact + oil) to determine gene expression changes of Kiss1 and Gnrh1 in the hypothalamus and protein expression of kisspeptin and GnRH in the different areas of the hypothalamus. In the intact + E-2 and OVX + E-2 rats on the day of the onset of puberty, GnRH-immunoreactive (ir) cell numbers decreased (P < 0.05) in the arcuate nucleus but were increased in the preoptic area; Kisspeptin-ir cells increased (P < 0.05) in the arcuate nucleus, periventricular nucleus, and preoptic area; no difference (P > 0.05) was found in the paraventricularis nucleus for GnRH-ir or kisspeptin-ir cells. Additionally, levels of Kiss1 and Gnrh1 messenger RNA in the hypothalamus were significantly higher (P < 0.05) in the OVX + E-2 or intact + E-2 rats than in the OVX + oil or intact + oil animals, respectively. In the OVX + oil rats, OVX significantly increased (P < 0.05) levels of Gnrh1 and Kiss1 messenger RNA and the expression of GnRH and kisspeptin in the hypothalamus compared to intact + oil animals. These results suggest that kisspeptin and GnRH play major roles in modulating the activity of estrogen circuits at the onset of puberty. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：1556 / 1564
页数：9
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