Functional Characterization of the Human Sweet Taste Receptor: High-Throughput Screening Assay Development and Structural Function Relation

被引：0
作者：
Li, Xiaodong [1 ]
Servant, Guy [1 ]
机构：
[1] Senomyx Inc, San Diego, CA 92121 USA
来源：
SWEETNESS AND SWEETENERS | 2008年 / 979卷
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human sweet taste receptor is a heteromeric complex of T1R2 and T1R3. We have developed a high throughput screening (HTS) assay for the receptor and demonstrated a tight correlation between the in vitro receptor activity and in vivo taste behavior. Furthermore, we developed a human-rat chimeric receptor system, and discovered different functional domains on both subunits of the receptor.
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页码：368 / 385
页数：18
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共 23 条
  • [1] Positional cloning of the mouse saccharin preference (Sac) locus
    Bachmanov, AA
    Li, X
    Reed, DR
    Ohmen, JD
    Li, SR
    Chen, ZY
    Tordoff, MG
    de Jong, PJ
    Wu, CY
    West, DB
    Chatterjee, A
    Ross, DA
    Beauchamp, GK
    [J]. CHEMICAL SENSES, 2001, 26 (07) : 925 - 933
  • [2] General pseudoreceptor model for sweet compounds: A semiquantitative prediction of binding affinity for sweet-tasting molecules
    Bassoli, A
    Drew, MGB
    Merlini, D
    Morini, G
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 2002, 45 (20) : 4402 - 4409
  • [3] Closure of the Venus flytrap module of mGlu8 receptor and the activation process:: Insights from mutations converting antagonists into agonists
    Bessis, AS
    Rondard, P
    Gaven, F
    Brabet, I
    Triballeau, N
    Prézeau, L
    Acher, F
    Pin, JP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) : 11097 - 11102
  • [4] Detection of sweet and umami taste in the absence of taste receptor T1r3
    Damak, S
    Rong, MQ
    Yasumatsu, K
    Kokrashvili, Z
    Varadarajan, V
    Zou, SY
    Jiang, PH
    Ninomiya, Y
    Margolskee, RF
    [J]. SCIENCE, 2003, 301 (5634) : 850 - 853
  • [5] Allosteric interactions between GB1 and GB2 subunits are required for optimal GABAB receptor function
    Galvez, T
    Duthey, B
    Kniazeff, J
    Blahos, J
    Rovelli, G
    Bettler, B
    Prézeau, L
    Pin, JP
    [J]. EMBO JOURNAL, 2001, 20 (09) : 2152 - 2159
  • [6] Allosteric modulators of group I metabotropic glutamate receptors: novel subtype-selective ligands and therapeutic perspectives
    Gasparini, F
    Kuhn, R
    Pin, JP
    [J]. CURRENT OPINION IN PHARMACOLOGY, 2002, 2 (01) : 43 - 49
  • [7] Putative mammalian taste receptors: A class of taste-specific GPCRs with distinct topographic selectivity
    Hoon, MA
    Adler, E
    Lindemeier, J
    Battey, JF
    Ryba, NJP
    Zuker, CS
    [J]. CELL, 1999, 96 (04) : 541 - 551
  • [8] Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste
    Jiang, PH
    Cui, M
    Zhao, BH
    Liu, Z
    Snyder, LA
    Benard, LMJ
    Osman, R
    Margolskee, RF
    Max, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (15) : 15238 - 15246
  • [9] Molecular genetic identification of a candidate receptor gene for sweet taste
    Kitagawa, M
    Kusakabe, Y
    Miura, H
    Ninomiya, Y
    Hino, A
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 283 (01) : 236 - 242
  • [10] Kniazeff J, 2002, J NEUROSCI, V22, P7352
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