Prevention of Staphylococcus epidermidis biofilm formation using electrical current

被引：11

作者：

Del Pozo, Jose L. ^{[1
,2
]}

Rouse, Mark S. ^{[2
]}

Euba, Gorane ^{[1
,2
]}

Greenwood-Quaintance, Kerryl E. ^{[2
,4
]}

Mandrekar, Jayawant N. ^{[3
]}

Steckelberg, James M. ^{[1
,2
]}

Patel, Robin ^{[1
,2
,4
]}

机构：

[1] Mayo Clin, Coll Med, Div Infect Dis, Dept Med, Rochester, MN 55905 USA

[2] Mayo Clin, Coll Med, Infect Dis Res Lab, Rochester, MN 55905 USA

[3] Mayo Clin, Coll Med, Dept Hlth Sci Res, Rochester, MN 55905 USA

[4] Mayo Clin, Coll Med, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA

来源：

JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS | 2014年 / 12卷 / 02期

关键词：

Electrical current; Staphylococcus epidermidis; Biofilm; Electricidal effect; CURRENT-INDUCED DETACHMENT; ANTIMICROBIAL AGENTS; BACTERIA;

D O I：

10.5301/jabfm.5000208

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A technique for the prevention of staphylococcal adhesion by electrical current exposure was investigated. Teflon coupons were exposed to a continuous flow of 10(3) cfu/ml Staphylococcus epidermidis with or without 2000 microA DC electrical current delivered by electrodes on opposite sides of a coupon, touching neither each other nor the coupon. A mean 3.46 (SD, 0.20) and 5.70 (SD, 1.03) log(10) cfu/cm(2) were adhered to the non-electrical current exposed coupons after 4 h and 24 h, respectively. A mean 2.46 (SD, 0.31) and 1.47 (SD, 0.73) log(10) cfu/cm(2) were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by graphite electrodes. A mean 2.21 (SD, 0.14) and 0.55 (SD, 0.00) log(10) cfu/cm(2) were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by stainless steel electrodes. Electrical current may be useful in the prevention of staphylococcal adhesion to biomaterials.

引用

页码：81 / 83

页数：3

共 13 条

[1] TESTING THE SUSCEPTIBILITY OF BACTERIA IN BIOFILMS TO ANTIBACTERIAL AGENTS [J].

ANWAR, H ;

DASGUPTA, MK ;

COSTERTON, JW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (11) :2043-2046

[2] Role of antibiofilm-antimicrobial agents in controlling device-related infections [J].

Aslam, Saima ;

Darouiche, Rabih O. .

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 2011, 34 (09) :752-758

[3] MECHANISM OF ELECTRICAL ENHANCEMENT OF EFFICACY OF ANTIBIOTICS IN KILLING BIOFILM BACTERIA [J].

COSTERTON, JW ;

ELLIS, B ;

LAM, K ;

JOHNSON, F ;

KHOURY, AE .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (12) :2803-2809

[4] The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis [J].

Del Pozo, Jose L. ;

Rouse, Mark S. ;

Euba, Gorane ;

Kang, Cheol-In ;

Mandrekar, Jayawant N. ;

Steckelberg, James M. ;

Patel, Robin .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (10) :4064-4068

[5] The Electricidal Effect: Reduction of Staphylococcus and Pseudomonas Biofilms by Prolonged Exposure to Low-Intensity Electrical Current [J].

del Pozo, Jose L. ;

Rouse, Mark S. ;

Mandrekar, Jayawant N. ;

Steckelberg, James M. ;

Patel, Robin .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (01) :41-45

[6] Effect of Electrical Current on the Activities of Antimicrobial Agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis Biofilms [J].

del Pozo, Jose L. ;

Rouse, Mark S. ;

Mandrekar, Jayawant N. ;

Sampedro, Marta Fernandez ;

Steckelberg, James M. ;

Patel, Robin .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (01) :35-40

[7] Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon [J].

Jucker, BA ;

Harms, H ;

Zehnder, AJB .

JOURNAL OF BACTERIOLOGY, 1996, 178 (18) :5472-5479

[8]

Poortinga AT, 2000, BIOTECHNOL BIOENG, V67, P117, DOI 10.1002/(SICI)1097-0290(20000105)67:1<117::AID-BIT14>3.0.CO

[9]

2-6

[10]

Pozo JL Del, 2007, 47 ANN INT C ANT AG

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