Novel Fluorescent Probe for Analysis of Hydroperoxides Based on Boron Dipyrromethane Fluorophore

被引：15

作者：

Inoue, Naoko ^{[1
]}

Suzuki, Yoshio ^{[1
]}

Yokoyama, Kenji ^{[1
,2
]}

Karube, Isao ^{[1
]}

机构：

[1] Tokyo Univ Technol, Katayanagi Inst, Natl Inst Adv Ind Sci & Technol, Res Ctr Adv Bion, Tokyo 1920982, Japan

[2] Natl Inst Adv Ind Sci & Technol, Res Ctr Adv Bion, Tsukuba, Ibaraki 3058562, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 05期

关键词：

BODIPY; hydroperoxide; lipid hydroperoxide; reagent; LIPID-PEROXIDATION; AROMATIC PHOSPHINES; DIPHENYL-1-PYRENYLPHOSPHINE; FLUOROMETRY; ELECTRODE; PLASMA; SENSOR;

D O I：

10.1271/bbb.80899

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new reagent, N-[2-(diphenylphosphino)ethyhl]-4(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (lambda(ex)/lambda(em) = 502nm/515nm) and reacts with bydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.

引用

页码：1215 / 1217

页数：3

共 15 条

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