Doripenem, Gentamicin, and Colistin, Alone and in Combinations, against Gentamicin-Susceptible, KPC-Producing Klebsiella pneumoniae Strains with Various ompK36 Genotypes

被引:34
作者
Clancy, Cornelius J. [1 ,2 ,4 ]
Hao, Binghua [1 ,2 ]
Shields, Ryan K. [1 ,2 ]
Chen, Liang [3 ]
Perlin, David S. [3 ]
Kreiswirth, Barry N. [3 ]
Nguyen, M. Hong [1 ,2 ]
机构
[1] Univ Pittsburgh, Pittsburgh, PA 15260 USA
[2] Univ Pittsburgh, Med Ctr, XDR Pathogen Lab, Pittsburgh, PA USA
[3] Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ 07103 USA
[4] Pittsburgh VA Healthcare Syst, Pittsburgh, PA USA
基金
美国国家卫生研究院;
关键词
BETA-LACTAMASES; MULTIPLEX PCR; PORIN; RESISTANT; BACTEREMIA; GENES;
D O I
10.1128/AAC.01949-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Gentamicin doses of 2 and 10 mu g/ml were bactericidal against 64% and 100%, respectively, of gentamicin-susceptible KPC-2-producing Klebsiella pneumoniae strains. Treatment with the combination of doripenem (8 mu g/ml) plus colistin (2 mu g/ml) was inferior to treatment with gentamicin (2 mu g/ml), doripenem-gentamicin, gentamicin-colistin, and doripenem-gentamicin-colistin against strains with glycine and aspartic acid insertions in OpmK36 porin at amino acid (aa) positions 134 and 135 (n = 9). Doripenem-colistin was comparable to other 2- or 3-drug regimens and superior to single drugs against wild-type/minor ompK36 mutants (n = 5). An algorithm incorporating ompK36 genotypes and susceptibility to gentamicin and doripenem may predict antimicrobial activity against KPC-producing K. pneumoniae.
引用
收藏
页码:3521 / 3525
页数:5
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