Optimizing RNA interference for application in mammalian cells

被引：35

作者：

Medema, RH ^{[1
]}

机构：

[1] Netherlands Canc Inst, Div Mol Biol, NL-1066 CX Amsterdam, Netherlands

来源：

BIOCHEMICAL JOURNAL | 2004年 / 380卷

关键词：

complementation analysis; mitosis; off target; secondary effect; small interfering RNA (siRNA); synchronization;

D O I：

10.1042/BJ20040260

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the last 2 years, the scientific community has rapidly embraced novel technologies that allow gene silencing in vertebrates. Ease of application, cost effectiveness and the possibilities for genome-wide reverse genetics have quickly turned this approach into a widely accepted, almost mandatory asset for a self-respecting laboratory in life sciences. This review discusses some of the recent technological developments that allow the application of RNAi (RNA interference) in mammalian cells. In addition, the advantages of applying RNAi to study cell cycle events and the emerging approaches to perform mutational analysis by complementation in mammalian cells are evaluated. In addition, common pitfalls and drawbacks of RNAi will be reviewed, as well as the possible ways to get around these shortcomings of gene silencing by small interfering RNA.

引用

页码：593 / 603

页数：11

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