Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae)

被引：3

作者：

Spironello, Mike

Rizvi, Leena

Currie, Douglas C.

Davy, Christina M.

Murphy, Robert W. ^{[1
]}

机构：

[1] Royal Ontario Museum, Dept Nat Hist, Toronto, ON M5S 2C6, Canada

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

black flies; genetic population structure; microsatellite; primers; sibling species; Simulium;

D O I：

10.1111/j.1755-0998.2009.02544.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven polymorphic microsatellite loci were isolated and developed from the black fly, Simulium negativum, a member of the Simulium arcticum sibling species complex. The observed heterozygosity of the 11 loci ranged from 0.03 to 0.83. The number of alleles per locus ranged from 8 to 19. Significant linkage disequilibria were encountered only for the primer pairs BF7-1 with BF7-5 and BF6-32 with BF7-16. Presumably, these microsatellite loci can be used to study genetic structure within the entire S. arcticum complex.

引用

页码：969 / 971

页数：3

共 10 条

[1]

Adler PH., 2004, The black flies (Simuliidae) of North America

[2] Inference of population structure using multilocus genotype data: dominant markers and null alleles [J].