Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells

被引：20

作者：

Mittag, Anna ^{[1
]}

Schneider, Thomas ^{[1
]}

Westermann, Martin ^{[2
]}

Glei, Michael ^{[1
]}

机构：

[1] Friedrich Schiller Univ Jena, Inst Nutr Sci, Dept Nutr Toxicol, Jena, Germany

[2] Friedrich Schiller Univ Jena, Electron Microscopy Ctr, Jena, Germany

来源：

ARCHIVES OF TOXICOLOGY | 2019年 / 93卷 / 06期

关键词：

Flow cytometry; HT29; cells; Magnesium oxide; Nanoparticles; Nanotoxicology; Oral exposure; MGO NANOPARTICLES; TITANIUM-DIOXIDE; CYTOTOXICITY; EXPOSURE; NANOTECHNOLOGIES; NANOMEDICINE; GENOTOXICITY; APOPTOSIS; DAMAGE; RATS;

D O I：

10.1007/s00204-019-02451-4

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Nanoparticles (NPs) are increasingly used in different consumer-related areas, for instance in food packaging or as additives, because of their enormous potential. Magnesium oxide (MgO) is an EU-approved food additive (E number 530). It is commonly used as a drying agent for powdered foods, for colour retention or as a food supplement. There are no consistent results regarding the effects of oral MgO NP uptake. Consequently, the aim of this study was to examine the effects of MgO NPs in the HT29 intestinal cell line. MgO NP concentrations ranged from 0.001 to 100 mu g/ml and incubation times were up to 24h. The cytotoxic and genotoxic potential were investigated. Apoptotic processes and cell cycle changes were analysed by flow cytometry. Finally, oxidative stress was examined. Transmission electron microscopy indicated that there was no cellular uptake. MgO NPs had no cytotoxic or genotoxic effects in HT29 cells and they did not induce apoptotic processes, cell cycle changes or oxidative stress.

引用

页码：1491 / 1500

页数：10