Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

被引：30
|
作者：
Nuutila, AM [1 ]
Hämäläinen, J [1 ]
Mannonen, L [1 ]
机构：
[1] VTT Biotechnol & Food Res, FIN-02044 Espoo, Finland
来源：
PLANT SCIENCE | 2000年 / 151卷 / 01期
关键词：
barley; Hordeum vulgare; mathematical models; medium optimization; polyembryogenesis; regeneration;
D O I：
10.1016/S0168-9452(99)00202-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：85 / 92
页数：8
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