Preservation and artificial insemination of sexed semen in sheep

被引：18

作者：

Evans, G ^{[1
]}

Hollinshead, FK ^{[1
]}

Maxwell, WMC ^{[1
]}

机构：

[1] Univ Sydney, Fac Vet Sci, Ctr Adv Technol Anim Genet & Reprod, Sydney, NSW 2006, Australia

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 2004年 / 16卷 / 04期

关键词：

cryopreservation; sperm sorting;

D O I：

10.1071/RD04032

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sperm-sexing technology using flow cytometry is in advanced stages of development for the sperm of several species. The sorting process could compromise sperm viability and sperm require specific handling procedures both before and after sorting to maintain the integrity and function of the sorted sperm. Standard freezing protocols have been modified for post-sorting cryopreservation of sperm and frozen sperm have been successfully thawed, sorted, refrozen and subsequently used to produce offspring. The relatively low numbers of available sorted sperm have, in some cases, led to modification of artificial insemination techniques to maximise efficiency of use. Multiple ovulation and embryo transfer, or in vitro fertilisation and associated technology, may lead to the more efficient use of sexed sperm.

引用

页码：455 / 464

页数：10

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